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Human TruStain FcX™, GMP

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Other Names
Fc Blocker, FcγR blocker, Fc inhibitor
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Human-TruStain-FcX_GMP_SOLUTION_060523
Human TruStain FcX™ treated (blue) or non-treated (red) THP-1 cells stained with an irrelevant mouse IgG2a PE mAb. Dotted histogram represents unstained cells.
  • Human-TruStain-FcX_GMP_SOLUTION_060523
    Human TruStain FcX™ treated (blue) or non-treated (red) THP-1 cells stained with an irrelevant mouse IgG2a PE mAb. Dotted histogram represents unstained cells.
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422305 50 tests $125
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422304 200 tests $395
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Description

Human Fc receptors (FcRs) are expressed on a variety of cells, such as monocytes, granulocytes, B cells and dendritic cells. The cells with FcR expression sometimes give false positive or false negative results of immunofluorescent staining due to the FcRs-mediated Ig Fc binding. Human TruStain FcX™ is specially formulated for blocking the FcR-involved unwanted staining without interfering with antibody-mediated specific staining of human cells. Human TruStain FcX™ is compatible with flow cytometric staining with anti-human CD16 (clone 3G8), CD32 (clone FUN-2), and CD64 (clone 10.1) antibodies.

Product Details
Technical data sheet

Product Details

Formulation
Human IgG in PBS containing 0.09% sodium azide
Storage & Handling
Store between 2°C-8°C
Application

FC - Quality tested
Recommended Usage

It is recommended to add 5 µl of Human TruStain FcX™ per million cells in 100 µl staining volume, mix and incubate at room temperature for 5-10 minutes prior to staining with antibody of interest. It is not necessary to wash cells between these blocking and immunostaining steps.

Application Notes

This buffer contains specialized human IgG in PBS containing 0.09% sodium azide. It is not recommended to be used for staining human IgG. Handle as biohazard agent.

Application References

(PubMed link indicates BioLegend citation)
  1. de la Cruz J, et al. 2011. J. Immunol. 186:2282. PubMed
  2. Luo L, et al. 2011. Exp Mol Pathol. 91:596. PubMed
  3. Szerlip NJ, et al. 2012. PNAS. 109:3041. PubMed
  4. Bromley SK, et al. 2013. J. Immunol. 190:970. PubMed
  5. Trujilo G, et al. 2013. J. Immunol. 191:848. PubMed
  6. Edin S, et al. 2013. PLoS One. 18:74982. PubMed
  7. Euzebio Alves VT, et al. 2013. Infect Immun. 81:4399. PubMed
  8. Borg ZD, et al. 2014. J. Immunol. 192:189. PubMed
  9. Klingenberg R, et al. 2014. Eur Heart J. PubMed
  10. Lines JL, et al. 2014. Cancer Res. 74:1924. PubMed
  11. Decker D, et al. 2014. Lupus. 23:802. PubMed
  12. Jitschin R, et al. 2014. Blood. 123:2663. PubMed
  13. Anderson ME Jr, et al. 2014. Otolaryngol Head Neck Surg. 150:880. PubMed
  14. Giurisato E, et al. 2014. Mol Biol Cell. 25:1769. PubMed
  15. Shi J, et al. 2014. Blood. 123:4015. PubMed
  16. Kusner LL, et al. 2014. PLoS One. 9:102231. PubMed
  17. Fujisawa D, et al. 2014. J Allergy Clin Immunol. 134:622. PubMed
  18. Bhardwaj N, et al. 2014. J Virol. 88:11108. PubMed
  19. Lieber S, et al. 2014. Mol Pharmacol. 87:162. PubMed
  20. Olagnier D, et al. 2014. PLoS Pathog. 10:1004566. PubMed
  21. Punyadee N, et al. 2015. J Virol. 89:1587. PubMed
  22. Vuong TT, et al. 2015. J Histochem Cytochem. 63:280. PubMed
  23. Klingenberg R, et al. 2015. Eur Heart J. 36:1041. PubMed
  24. Wilkinson-Ryan I, et al. 2015. PLoS One.10:125851. PubMed

Antigen Details

Molecular Family
Fc Receptors
Gene ID
NA
Go To Top Version: 3    Revision Date: 02/20/2024

For Research Use Only. Suitable for ex vivo cell processing. Not for injection or diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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