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LEAF™ Purified anti-mouse IL-1β Antibody

Pricing & Availability
Clone
Poly5158 (See other available formats)
Regulatory Status
RUO
Other Names
Interleukin-1β, Catabolin, Hematopoietin-1 (H1), IFNβ-inducing factor, Interleukin-β, Osteoclast activating factor (OAF)
Isotype
Goat Polyclonal IgG
Ave. Rating
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Product Citations
publications
Poly5158_LEAF_IL-1B_Antibody_1_020821
Recombinant mouse IL-1β (Cat. No. 575102) (black circles) induces proliferation on D10.G4.1 mouse helper T cells. LEAF™ purified anti-mouse IL-1β antibody (Poly5158) (purple squares) neutralizes the cell proliferation induced by recombinant mouse IL-1β (Cat. No. 575102) at 0.05 ng/mL on D10.G4.1 cells in a dose-dependent manner whereas the GoInVivo™ purified goat IgG isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 2.0 - 10 µg/mL.
  • Poly5158_LEAF_IL-1B_Antibody_1_020821
    Recombinant mouse IL-1β (Cat. No. 575102) (black circles) induces proliferation on D10.G4.1 mouse helper T cells. LEAF™ purified anti-mouse IL-1β antibody (Poly5158) (purple squares) neutralizes the cell proliferation induced by recombinant mouse IL-1β (Cat. No. 575102) at 0.05 ng/mL on D10.G4.1 cells in a dose-dependent manner whereas the GoInVivo™ purified goat IgG isotype ctrl antibody (gray triangles) does not have the effect. ND50 range: 2.0 - 10 µg/mL.
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515805 100 µg $417
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Description

IL-1 refers to two proteins, IL-1α and IL-1β which are the products of distinct genes, but which are recognized by the same cell surface receptors. IL-1β is a potent immuno-modulator which mediates a wide range of immune and inflammatory responses including the activation of B and T cells. The Poly5158 antibody reacts with the precursor and mature secreted forms of mouse IL-1β.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Polyclonal
Host Species
Goat
Immunogen
Recombinant mouse IL-1β
Formulation
0.2 µm filtered in phosphate-buffered solution, pH 7.2, containing no preservative.
Endotoxin Level
Less than 0.1 EU/µg of the protein (< 0.01 ng/µg of the protein) as determined by the LAL test.
Preparation
The LEAF™ (Low Endotoxin, Azide-Free) antibody was purified by affinity chromatography.
Concentration
The antibody is bottled at the concentration indicated on the vial. To obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.
Storage & Handling
Upon receipt, store frozen at -20°C. Make small volume aliquots if needed and avoid repeated freeze-thaw cycles to prevent denaturing the antibody.
Application

Neut - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by neutralization of proliferation induced by recombinant mouse IL-1β (Cat. No. 575102) at 0.05 ng/mL on D10.G4.1 mouse helper T cells. ND50 range: 2.0 - 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

ELISA Detection: The biotinylated Poly5158 antibody is useful as a detection antibody for a sandwich ELISA assay, when used in conjunction with purified B122 antibody (Cat. No. 503502) as the capture antibody.

RRID
AB_2892476 (BioLegend Cat. No. 515805)

Antigen Details

Structure
Cytokine; 17.5 kD (Mammalian)
Bioactivity
Stimulates T cells, B cells, proliferation/activation of NK cells, fibroblasts, thymocytes, glioblastoma cells, astroglia, microglia; monocyte transition from IL-1β to IL-1α when matured to macrophages
Cell Sources
Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma
Cell Targets
B cells, T cells, monocytes
Receptors
Type I IL-1R (CDw121a), Type II IL-1R (CDw121b)
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. Bomford R, et al. Eds. 1989. Interleukin-1 inflammation and disease. Elsevier New York.
3. Brazel D, et al. 1991. Biotechnol. Ther. 2:241.
4. Dinarello C et al. 1996. Blood 87:2095.

Regulation
Upregulated by TNF-α, IFN-α, IFN-β, IFN-γ, bacterial endotoxins, viruses, mitogens, antigens; downregulated by IL-6, lipoproteins, lipids, and α2-macroglobulin
Gene ID
3553 View all products for this Gene ID
UniProt
View information about IL-1beta on UniProt.org

Related FAQs

Do you guarantee that your antibodies are totally pathogen free?

BioLegend does not test for pathogens in-house aside from the GoInVivo™ product line. However, upon request, this can be tested on a custom basis with an outside, independent laboratory.

Does BioLegend test each Ultra-LEAF™ antibody by functional assay?

No, BioLegend does not test Ultra-LEAF™ antibodies by functional assays unless otherwise indicated. Due to the possible complexities and variations of uses of biofunctional antibodies in different assays and because of the large product portfolio, BioLegend does not currently perform functional assays as a routine QC for the antibodies. However, we do provide references in which the antibodies were used for functional assays and we do perform QC to verify the specificity and quality of the antibody based on our strict specification criteria.

Does BioLegend test each Ultra-LEAF™ antibody for potential pathogens?

No, BioLegend does not test for pathogens in-house unless otherwise indicated.  However, we can recommend an outside vendor to perform this testing as needed.

Have you tested this Ultra-LEAF™ antibody for in vivo or in vitro applications?

We don't test our antibodies for in vivo or in vitro applications unless otherwise indicated. Depending on the product, the TDS may describe literature supporting usage of a particular product for bioassay. It may be best to further consult the literature to find clone specific information.

Go To Top Version: 3    Revision Date: 08/29/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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