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LEGEND MAX™ Human NGAL (Lipocalin-2) ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
Lipocalin2, LCN2, Siderocalin
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Product Citations
publications
NGAL_Human_ELISA_Kit_LegMax_051315
  • NGAL_Human_ELISA_Kit_LegMax_051315
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443407 1 Pre-coated Plate $462
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Description

Neutrophil Gelatinase-Associated Lipocalin (NGAL), also known as Lipocalin-2, Siderocalin, 24p3, Uterocalin, or Neu-related lipocalin, is a member of the lipocalin family. It is a secreted protein (25 kD), which captures bacterial siderophores that bind iron with high affinity. NGAL predominantly exists in monomeric form with a small portion in homodimeric and homotrimeric forms. It can also form a heterodimer with MMP-9 and a heterotrimer with MMP-9 and TIMP-1. Human NGAL shares 62% amino acid sequence identity with mouse NGAL.

The function of NGAL is diverse and continues to be studied. NGAL acts as a potent anti-bacterial agent via siderophore-mediated iron depletion. NGAL knockout mice exhibit decreased ability to combat bacterial infection. NGAL has been used as an early predictive and prognostic biomarker for acute and chronic kidney injuries. It also has been associated with breast, lung, colon and pancreatic cancer, having diverse, context-dependent effects during tumor development. This may involve cell growth, survival, migration, invasion and angiogenesis. When NGAL form a complex with MMP-9, it protects MMP-9 from degradation, thereby preserving MMP-9’s enzymatic activity, which is involved in various cancers.

The LEGEND MAX™ Human NGAL ELISA kit is a Sandwich Enzyme-Linked Immunosorbent Assay (ELISA) with a 96-well strip plate that is pre-coated with a mouse monoclonal anti-human NGAL antibody. The Detection Antibody is a biotinylated mouse monoclonal anti-human NGAL antibody. This kit is specifically designed for the accurate quantitation of human NGAL from cell culture supernatant, serum, plasma, urine, saliva, and other biological fluids. This kit is analytically validated with ready-to-use reagents.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-Human NGAL Pre-coated 96 well Strip Microplate
  • Human NGAL Detection Antibody
  • Human NGAL Standard
  • Avidin-HRP
  • Assay Buffer B
  • Wash Buffer (20X)
  • Susbstrate Solution D
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Human
Application

ELISA
Product Citations
  1. Zhang Y, et al. 2022. J Clin Lab Anal. 36:e24526. PubMed
  2. Lundquist H, et al. 2022. J Innate Immun. :1. PubMed
  3. Ruff WE, et al. 2019. Cell Host Microbe. 26:100. PubMed
  4. Li G, et al. 2021. Gut Microbes. 13:1968257. PubMed
Sensitivity
16.4 pg/mL
Standard Range
156 - 10,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1µl to 1,000 µl
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity, Signal Transduction
Gene ID
3934 View all products for this Gene ID
UniProt
View information about NGAL on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 08/05/2021

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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