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LEGEND MAX™ Mouse TNF-α ELISA Kit

Pricing & Availability
Regulatory Status
RUO
Other Names
TNF-a ELISA Kit
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Product Citations
publications
TNF-a 061809
  • TNF-a 061809
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430907 1 Pre-coated Plate $345
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Description

TNF-a is secreted by macrophages, monocytes, neutrophils, T-cells (principally CD4+), and NK-cells. Many transformed cell lines also secrete TNF-a. TNF-a forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-a has also been described. TNF-a binding to surface receptors elicits a wide array of biologic activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Anti-Mouse TNF-α Pre-coated 96-well Strip Microplate
  • Mouse TNF-α Dectection Antibody
  • Mouse TNF-α Standard
  • Matrix A (for serum and plasma samples only)
  • Avidin-HRP B
  • Assay Buffer A
  • Wash Buffer (20X)
  • Substrate Solution D
  • Stop Solution
  • Plate Sealers

Product Details

Verified Reactivity
Mouse
Application

ELISA
Product Citations
  1. McKnight Q, et al. 2020. J Bone Miner Res. 35:1352. PubMed
  2. Lliberós C, et al. 2021. Front Cell Dev Biol. 8:628473. PubMed
  3. Hu CH, et al. 2022. Small Methods. 6:e2100763. PubMed
  4. Wiesenthal AA, et al. 2023. Biology (Basel). 12:. PubMed
  5. Sun Q, et al. 2023. Transl Neurosci. 14:20220273. PubMed
  6. Yokozeki Y, et al. 2021. BMC Musculoskelet Disord. 22:634. PubMed
  7. Ma D, et al. 2021. Sci Adv. :7. PubMed
  8. Pan W, et al. 2020. Exp Ther Med. 19:1695. PubMed
  9. Du L, et al. 2022. Aging (Albany NY). 14:6626. PubMed
  10. Minati MA, et al. 2021. Antioxidants (Basel). 10:. PubMed
  11. Yin Y, et al. 2022. Front Pharmacol. 13:882924. PubMed
  12. Tao W, et al. 2020. Sci Transl Med. 12: . PubMed
  13. Wu D, et al. 2022. Cell Death Dis. 13:731. PubMed
  14. Wu H, et al. 2019. Autophagy. :1. PubMed
  15. Zhang P, et al. 2022. BMC Med. 20:435. PubMed
  16. Chang LC, et al. 2020. Biomedicines. 8:00. PubMed
  17. Xu X, et al. 2018. Cell. 175:1336. PubMed
  18. Lian H, et al. 2021. Nat Microbiol. 6:658. PubMed
  19. Xiang K, et al. 2022. Front Immunol. 13:814319. PubMed
  20. Tian M, et al. 2020. Cell Rep. 108297:33. PubMed
  21. Chen H, et al. 2021. Invest Ophthalmol Vis Sci. 62:11. PubMed
  22. Kueanjinda P, Palaga S 2015. Sci Rep. 5: 12784. PubMed
  23. Yu WQ, et al. 2019. J Zhejiang Univ Sci B. 20:355. PubMed
  24. Tung YT, et al. 2021. Antioxidants (Basel). 10:. PubMed
  25. Lu Q, et al. 2022. Biomed Res Int. 2022:9427076. PubMed
  26. Lei G, et al. 2017. Antimicrob Agents Chemother. 10.1128/AAC.01226-17. PubMed
  27. Chang C, et al. 2021. Mol Med Rep. :23. PubMed
  28. Su L, et al. 2019. EMBO J. 38:. PubMed
  29. Wang X, et al. 2022. Front Cell Infect Microbiol. 12:839592. PubMed
  30. Zhang SJ, et al. 2021. Adv Sci (Weinh). 8:2003410. PubMed
  31. Huang WC, et al. 2022. Sci Rep. 12:3612. PubMed
Sensitivity
1.5 pg/ml
Standard Range
15.6-1,000 pg/mL
Materials Not Included
  • Microplate reader able to measure absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 1 µL to 1,000 µL
  • Deionized water
  • Wash bottle or automated microplate washer
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Shaker
  • Polypropylene vials

Antigen Details

Cell Sources
Activated monocytes, neutrophils, macrophages, T cells, B cells, NK cells, LAK cells
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
21926 View all products for this Gene ID
UniProt
View information about TNF-alpha on UniProt.org

Related FAQs

In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?

We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.

I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?

The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.

For some of your ELISA kits, why do my serum samples require dilution with assay buffer?

In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.

Go To Top Version: 2    Revision Date: 09/11/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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