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- Regulatory Status
- RUO
- Other Names
- Interferon beta 1
- Ave. Rating
- Submit a Review
- Product Citations
- publications
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 449507 | 1 Pre-coated Plate | $655 | ||||
IFN-β is a type I class of interferon produced by macrophages, dendritic cells, endothelial cells and primarily fibroblasts. It plays a significant antiviral activity role within the innate immune response to infection, cell differentiation and anti-tumor defenses. Once secreted, it binds to a homologous receptor complex and activates the Jak/STAT pathway, resulting in upregulating genes that inhibit viral infection and regulate MHC class 1 antigens. Multiple sclerosis is characterized by a deficiency of IFN beta 1 and is commonly been used as an injectable treatment.
Product DetailsKit Contents
- Kit Contents
-
- Human IFN-β Pre-Coated Plate
- Human IFN-β Detection Antibody
- Human IFN-β Standard
- Assay Buffer B
- Streptavidin-Polymer HRP
- Wash Buffer (20X)
- Substrate Solution D
- Stop Solution
- Plate Sealers
Product Details
- Verified Reactivity
- Human
- Application
-
ELISA
- Sensitivity
- 0.91 ± 0.16 pg/mL
- Standard Range
- 2.34 - 150 pg/mL
- Materials Not Included
-
- Microwell plates: BioLegend Cat. No. 423501 is recommended
- Wash Buffer: BioLegend Cat. No. 421601 is recommended, or PBS + 0.05% Tween-20
- Stop Solution: BioLegend Cat. No. 423001 is recommended, or acid solution, e.g. 2N H2SO4
- Plate Sealers: BioLegend Cat. No. 423601 is recommended
- PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4 , 0.2 g KH2PO4 , 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 μm filtered
- Deionized (DI) water
- A microplate reader capable of measuring absorbance at 450 nm
- Adjustable pipettes to measure volumes ranging from 2 μL to 1 mL
- Wash bottle or automated microplate washer
- Log-Log graph paper or software for data analysis
- Tubes to prepare standard dilutions
- Timer
Antigen Details
- Cell Type
- B cells, Dendritic cells, Endothelial cells, Fibroblasts, Macrophages
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Proliferation and Viability, COVID-19, Innate Immunity, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Innate Immune Signaling
- Gene ID
- 3456 View all products for this Gene ID
- UniProt
- View information about IFN-beta on UniProt.org
Related FAQs
- In your LEGEND MAX™ ELISA Kits, there is a step that calls for washing the plates before adding sample. What is the purpose of this step?
-
We typically use a stabilizer for pre-coated plates. The additional washing step is designed to remove these components before you start the assay. If you do not perform the washing, the effect on assay performance is negligible.
- I have multiple LEGEND MAX™ ELISA kits that I want to run simultaneously. Can I use the same wash buffer for all the kits?
-
The wash buffer provided in all our LEGEND MAX™ kits is the same and the part numbers on the wash buffer bottles in these kits should be identical. For ELISA MAX™ Deluxe and ELISA MAX™ Standard Sets, we provide a recipe for the wash buffer on each kit’s technical data sheet. This recipe is the same for all ELISA MAX™ sets.
- For some of your ELISA kits, why do my serum samples require dilution with assay buffer?
-
In some cases, dilution with assay buffer is required to minimize the matrix difference between the samples and the standards to achieve better accuracy.
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