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LEGENDplex™ Mouse Proinflammatory Chemokine Panel (13-plex) with V-bottom Plate

Pricing & Availability
Regulatory Status
RUO
Other Names
CCL5 (RANTES), CCL20 (MIP-3α), CCL11 (Eotaxin-1), CCL17 (TARC), CXCL1 (KC), CCL2 (MCP-1), CXCL9 (MIG), CXCL10 (IP-10), CCL3 (MIP-1α), CCL4 (MIP-1β), CXCL13 (BLC), CXCL5 (LIX), CCL22 (MDC)
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Product Citations
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Cat # Size Price Quantity Save
740451 100 tests $1761
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Description

Chemotactic cytokines or chemokines play pivotal roles in various processes such as immune surveillance, organ development, angiogenesis, and immune responses. Expression profiling of chemokines, especially those involved in inflammation and immune disorders, is important in achieving a deeper understanding of disease states.


The Mouse Proinflammatory Chemokine panel is a multiplex bead-based assay panel, using fluorescence-encoded beads suitable for use on various flow cytometers. This panel allows simultaneous quantification of 13 Mouse chemokines, including MCP-1 (CCL2), RANTES (CCL5), IP-10 (CXCL10), Eotaxin (CCL11), TARC (CCL17), MIP-1α (CCL3), MIP-1β (CCL4), MIG (CXCL9), MIP-3α (CCL20), LIX (CXCL5), KC (CXCL1), BLC (CXCL13) and MDC (CCL22). This assay panel provides higher detection sensitivities and broader dynamic ranges than traditional ELISA methods. The panel has been validated by detecting expected changes in biological samples.

A Mix and Match system is available to customize assays for specificities within this proinflammatory chemokine panel. 

Product Details
View Current Manual (pdf)

Kit Contents

Kit Contents
  • Setup Beads: PE Beads 
  • Setup Beads: Raw Beads 
  • Capture Beads 
  • Detection Antibodies 
  • Standard Cocktail, Lyophilized 
  • SA-PE 
  • Matrix B, Lyophilized 
  • Assay Buffer 
  • Wash Buffer 
  • V-bottom Plate 
  • Plate Sealers 

Product Details

Verified Reactivity
Mouse
Application

Multiplex
Learn more about LEGENDplex™ at biolegend.com/legendplex

Download the LEGENDplex™ software here.
Application References

(PubMed link indicates BioLegend citation)
  1. Gobert GN, et al. 2015. PLoS. Negl. Trop. Dis. 5:e0003760. PubMed.
  2. Sabari BR, et al. 2015. Mol. Cell 58:203. PubMed.
Product Citations
  1. Liao H, et al. 2023. Bioeng Transl Med. 8:e10357. PubMed
  2. Cui P, et al. 2023. J Neuroinflammation. 20:63. PubMed
  3. Monaghan KL, et al. 2023. Front Immunol. 14:1117828. PubMed
  4. Rao E, et al. 2021. Sci Immunol. 6:. PubMed
  5. Li Y, et al. 2020. Cell Rep. 30:1753. PubMed
  6. Sugimoto C, et al. 2022. Elife. 11:. PubMed
  7. Chakraborty S, et al. 2022. Sci Transl Med. . PubMed
  8. Gautam A, et al. 2020. Front Microbiol. 1713:11. PubMed
  9. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  10. Bunting MD, et al. 2022. Sci Adv. 8:eabk3327. PubMed
  11. Liang J, et al. 2020. Sci Adv. 6:eabc3646. PubMed
  12. Wang J, et al. 2022. J Clin Invest. :. PubMed
  13. Monaghan KL, et al. 2020. J Vis Exp. . PubMed
  14. Faget J, et al. 2017. Cell Rep. 3.090277778. PubMed
  15. Jiang X, et al. 2022. J Immunother Cancer. 10:. PubMed
  16. Farris BY, et al. 2019. Immun Inflamm Dis. 0.518055556. PubMed
  17. Simon Davis DA, et al. 2022. PLoS One. 17:e0264631. PubMed
  18. Boyce M, et al. 2022. Cells. 11:. PubMed
  19. Schmit T, et al. 2022. Cell Rep. 38:110456. PubMed
Materials Not Included
  • Flow Cytometer
  • Pipettes and Tips
  • Reagent Reservoirs for Multichannel Pipettes
  • Polypropylene Microfuge Tubes
  • Vortex Mixer
  • Sonicator
  • Plate Shaker
  • Centrifuge capable of spinning 96-well plate (if using V-bottom plates)
  • Manual

Related FAQs

If I don't have a vacuum, how do I remove the liquid from my plate?

If you do not have a vacuum, the assay should be run in a V-bottom plate. After centrifugation using a swinging-bucket rotor with a plate adaptor, you can remove the liquid by flicking the plate quickly, dumping the contents into a sink, and patting it dry carefully on a stack of clean paper towels without losing the beads. Alternatively, you can remove the liquid by using a pipette.

Should I perform the assay with the filter plates or with V-bottom plates?

Filter plates or V-bottom plates have been included in some kits for your convenience. A vacuum filtration unit is required to work with the filter plates. However, if you don’t have access to a vacuum manifold or if you prefer, then you can use the V-bottom plates and follow the recommended assay protocols for the type of plates you choose. All plates should be made from low binding polypropylene. Polystyrene ELISA or cell culture plates should not be used.

After I finish the staining process, how long can I wait before reading my LEGENDplex™ samples?

The samples can be kept overnight at 4°C while being protected from exposure to light and be read the next day. There may be a decrease in signal, but overall, the assay results should not be affected. Storing the samples for extended periods of time is not recommended, as it could lead to further reductions in signal.

What is the shelf life of LEGENDplex™ kits?

LEGENDplex™ kits are guaranteed for 6 months from the date of receipt, but may have a shelf life of up to 2 years from the date of manufacture.

Is special software required for data analysis?

Typically flow cytometers generate output files in FCS format (e.g. FCS 2.0, 3.0, or 3.1) and in some cases in list mode file format (LMD). Other software may be available to analyze FCS files. Data generated using LEGENDplex™ kits can be analyzed using the freely available LEGENDplex™ data analysis software. Please check our website for the most updated versions of the software.

Go To Top Version: 8    Revision Date: 06/14/2023

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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