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Lymphopure™

Pricing & Availability
Regulatory Status
RUO
Other Names
Density gradient medium
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Product Citations
publications
  • 2_lymphopure_FC_1and2and3_111617
    Forward and side scatter profile of human lymphocytes after peripheral blood centrifugation with Lymphopure™ (top dot plot), an equivalent product (middle plot), or no separation (bottom plot, lysed blood).
  • 3_lymphopure_FC_4and5_111617
    Histogram overlay shows CD3 or CD15 profile on total cells from lysed peripheral human blood before (dotted histogram) and after Lymphopure™ (purple tinted histogram) or equivalent product (orange open histogram) centrifugation. CD3 is a molecule expressed by T cells, which are recovered with the use of the isolation media. CD15 is a molecule highly expressed by granulocytes, which are absent after isolation with Lymphopure™.
  • 4_lymphopure_Diagram_111617
    Diagram showing whole blood cell separation using Lymphopure™. Blood is diluted with saline buffer and carefully layered over the medium in a conical tube, avoiding disturbing the interphase. Following centrifugation, at the appropriate speed with no braking, well-defined phases can be observed: 1-plasma, 2-interphase with mononuclear cells, 3-centrifugation medium, and 4-erythrocytes and granulocytes. Cells of interest can be aspirated from the interphase.
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426201 250 mL $89
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426202 500 mL $153
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Description

Lymphopure™ is a ready-to-use filtered and endotoxin tested solution for the isolation of mononuclear cells (MNCs) from a cell suspension. The solution is formulated at a density that allows separation of MNCs from other cells in blood such as granulocytes and erythrocytes. Granulocytes and erythrocytes will sediment after centrifugation.

Product Details
Technical data sheet

Product Details

Formulation
Density gradient medium for the isolation of mononuclear cells formulated at a density of 1.077 ± 0.001 g/mL, and osmolality of 290 ± 15 mOsm.
Storage & Handling
Upon receipt, store at room temperature protected from light.
Application

Cell Separation - Quality tested
Recommended Usage

Watch a video protocol.

  1. Collect blood into a tube containing anti-coagulant (EDTA, heparin)
  2. Dilute the blood with an equal volume of phosphate-buffered saline or similar suitable medium.
  3. Mix Lymphopure™ by inverting the bottle several times.
  4. Aliquot half the amount of Lymphopure™ into a tube. For every ml of diluted blood, add 0.5 ml of Lymphopure™.
  5. Carefully layer the diluted blood on top of Lymphopure™. Avoid mixing blood with Lymphopure™.
  6. Centrifuge at 800 x g for 20 minutes at room temperature (15 - 25°C) without braking. If the blood has been stored for more than 2 hours, centrifuge for 30 minutes.
  7. After centrifugation, mononuclear cells (MNCs) form a defined cell layer at the plasma: Lymphopure™ interface.
  8. Collect the MNC layer at the interface without disturbing the other fractions.
  9. Wash MNCs with medium.
Application References

(PubMed link indicates BioLegend citation)
  1. Böyum A. 1968. Scand. J. Clin. Lab. Invest. Suppl. 97:9-29.
  2. Böyum A. 1968. Scand. J. Clin. Lab. Invest. Suppl. 97:77-89.
  3. Böyum A. 1964. Nature. 204:793-4.
  4. Thorsby E, Bratile A. 1970. Histocompatibility Testing 1970,ed. P.I. Terasaki. 655-6.
  5. Ting A, Morris PJ. 1971. Vox. Sang. 20(6)561-3.

Antigen Details

Gene ID
NA
Go To Top Version: 2    Revision Date: 03/23/2018

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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