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- Regulatory Status
- RUO
- Other Names
- Liver Sinusoidal Endothelial Cell Isolation Kit, Liver Non-Parenchymal Cells, Mouse Liver CD146 Cell Isolation, Liver CD146 Positive Cells, Liver Sinusoidal Endothelial Cells
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- Product Citations
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Mouse cell suspensions from liver non-parenchymal cells (LNPCs) were prepared for the positive isolation of CD146+ LSECs using the MojoSort™ Mouse CD146 (LSEC) Selection Kit with a MojoSort™ Magnet (Cat. Nos. 480019/480020). After separation, the cells were stained with anti-mouse CD146 (clone ME-9F1) APC (Cat. No. 134711) and anti-mouse CD45 (clone 30-F11) PE (Cat. No. 103105). Doublets and dead cells were excluded from analysis.
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 480171 | 10 tests | $280 | ||||
| 480172 | 100 tests | $750 | ||||
Mouse CD146+ liver sinusoidal endothelial cells are either selected or depleted by incubating your sample with the anti-mouse CD146 Antibody Cocktail followed by the Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. These are the labeled CD146+ LSECs of interest; do not discard. After collection of the CD146+ cells, downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
-
For Cat# 480171:
- 1 vial containing 100 μL of anti-mouse CD146 Antibody Cocktail
- 1 vial containing 100 μL of Streptavidin Nanobeads
For Cat# 480172:
- 1 vial containing 1 mL of anti-mouse CD146 Antibody Cocktail
- 1 vial containing 1 mL of Streptavidin Nanobeads
Product Details
- Verified Reactivity
- Mouse
- Formulation
-
Cocktail: Phosphate buffer solution containing 0.09% sodium azide, 0.5% BSA, 5% HPCD, pH 7.2.
Particle: Aqueous solution containing BSA and 0.05% sodium azide. - Preparation
-
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions. The solution is free of unconjugated biotin.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads. - Storage & Handling
- All components should be stored undiluted between 2°C and 8°C.
- Application
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Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
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10 μL of anti-mouse CD146 Antibody Cocktail for 1 x 107 cells in 100 μL of buffer.
10 μL of Streptavidin Nanobeads for 1 x 107 cells in 100 μL of buffer. - Application Notes
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This kit is designed for the positive selection of CD146+ LSECs from mouse liver.
Test sizes/starting cell capacity:- The 10 and 100 test sizes provide enough reagents for approximately 2 x 108 and 2 x 109 liver non-parenchymal cells (LNPCs), respectively.
Optional:
- To futher increase purity of CD146+ cells from mouse liver, it is recommended to harvest LNPCs from PBS-perfused tissue.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: N/A
Nanobead dilution to use in column: N/A
Antigen Details
- Gene ID
- NA
- UniProt
- View information about CD146 on UniProt.org
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
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MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.
MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.
- What is the size of your magnetic particles?
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MojoSort Nanobeads: the average diameter is approximately 130 nm.
MojoSort Microbeads: the average diameter is approximately 1 μm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
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MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
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Please contact our technical service team for further assistance.
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