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MojoSort™ Streptavidin Nanobeads

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Regulatory Status
RUO
Other Names
SAV
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Product Citations
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StrepNano_1_051215
A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was prepared to isolate CD14+ cells using the Streptavidin Nanobeads. Cells were stained with CD14 (clone M5E2) FITC.
  • StrepNano_1_051215
    A single cell suspension from human peripheral blood mononuclear cells (PBMCs) was prepared to isolate CD14+ cells using the Streptavidin Nanobeads. Cells were stained with CD14 (clone M5E2) FITC.
  • StrepNano_2_051215
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480015 100 µL $113
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480016 1 mL $330
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Description

Streptavidin Nanobeads can be used for positive or negative selection of targeted cells with biotin-conjugated antibodies. For positive selection, the magnetically labeled fraction is retained by the use of a magnetic separator. For negative selection, the untouched cells are collected by decanting the liquid in a clean tube. Either positive or negative selection cells can be used for downstream applications such as functional assays, gene expression, phenotypic characterization, etc.

MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.

Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.

Product Details
Technical data sheet

Kit Contents

Kit Contents

For Cat. No. 480015:

  • 1 vial containing 100 µL of anti-Streptavidin Nanobeads

For Cat. No. 480016:

  • 1 vial containing 1 mL of anti-Streptavidin Nanobeads

Product Details

Formulation
Aqueous solution containing BSA and 0.05% sodium azide.
Preparation
Streptavidin-conjugated particles: protein-coated magnetic nanobeads.
Storage & Handling
Streptavidin Nanobeads should be stored undiluted between 2°C and 8°C.
Application

Cell Separation (MojoSort™) - Quality tested
Recommended Usage

The concentration of Streptavidin Nanobeads to be used should be optimized by end users.

Application Notes

The Streptavidin Nanobeads are designed for the positive or negative isolation of cells when used with biotin-conjugated antibodies or similar reagents.

Nanobead dilution to use in column: To be optimized by end users

Additional Product Notes

View more applications data using this product to isolate mouse T and B cells and microglia in our Scientific Poster Library.


Watch a Scientific Poster video of this product.

Application References

(PubMed link indicates BioLegend citation)
  1. Guo H, et al. 2016. PLoS One. 11(3):e0150809. (PubMed)
Product Citations
  1. Guo H, Cooper S, Friedman A, et al. 2017. PLoS One. 10.1371/journal.pone.0150809. PubMed
  2. Chanda MK, et al. 2022. J Immunol Methods. 506:113290. PubMed
  3. Gargaro M, et al. 2022. Immunity. 55:1032. PubMed
  4. Marié IJ, et al. 2022. Bio Protoc. 12:e4438. PubMed
  5. Ding L, et al. 2023. Immunology. 168:49. PubMed
  6. Slusarczyk P, et al. 2023. Elife. 12: . PubMed
  7. Huang P, et al. 2022. Nat Genet. 54:1417. PubMed
  8. Nazarov K, et al. 2023. Int J Mol Sci. 24:. PubMed
  9. Lukas H, et al. 2023. National Library of Medicine. . 120(33): e2300343120. PubMed
  10. Samra T, et al. 2023. Int J Mol Sci. :24. PubMed
  11. Biswas S, et al. 2024. Immunology. 534:171. PubMed
  12. Nazarov K, et al. 2023. Cells. :12. PubMed
  13. Downes DJ, et al. 2021. Nat Commun. 0.86875. PubMed
  14. Mitchell MI, et al. 2021. J Extracell Vesicles. 10:e12110. PubMed
  15. Rogers KJ, et al. 2021. J Leukoc Biol. 109:309. PubMed
  16. Dietmar Herndler‐Brandstetter et al. 2018. Immunity. 48(4):716-729 . PubMed
  17. Song J, et al. 2021. Cancer Commun (Lond). 41:867. PubMed
  18. Knittel J, et al. 2022. STAR Protoc. 3:101632. PubMed
  19. Zeis P, et al. 2020. Immunity. 53:775. PubMed
  20. Laubreton D, et al. 2020. Viruses. 12:00. PubMed
  21. Aoto M, et al. 2019. FEBS Open Bio. 9:291. PubMed
  22. Fujimoto M, et al. 2022. Nat Commun. 13:5408. PubMed
  23. Kenney D, et al. 2022. Methods Mol Biol. 2580:211. PubMed
  24. Wang X, et al. 2022. Elife. 11:. PubMed
  25. Oudelaar AM, et al. 2020. Nat Commun. 2.348611111. PubMed
  26. Waldman MM, et al. 2022. Front Immunol. 13:856977. PubMed
  27. Celias DP, et al. 2022. STAR Protoc. 3:101464. PubMed

Antigen Details

Gene ID
NA

Related FAQs

Is there a way to detach your magnetic particles from the cell surface?

MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.

 

MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.

What is the size of your magnetic particles?

MojoSort Nanobeads: the average diameter is approximately 130 nm.

 

MojoSort Microbeads: the average diameter is approximately 1 μm.

Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?

MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems.  Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.

What antibodies are present in the depletion cocktails provided for isolation kits?

Please contact our technical service team for further assistance.

Go To Top Version: 1    Revision Date: 05/14/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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