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Human neutrophils were isolated from whole blood using the MojoSort™ Whole Blood Human Neutrophil Cell Isolation Kit. Cells were stained with CD16 (clone 3G8) PE and CD66b (clone G10F5) APC. Dead cells were excluded by Helix NP™ Blue. Final purity and yield may vary between different donors. Left plot: CD16+/CD66b+ cells after isolation. Right plot: Cells before isolation
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 480152 | 100 tests | $522 | ||||
Human non-neutrophil cells are depleted by incubating of sample with the biotinylated antibody cocktail followed by incubation with magnetic Streptavidin Nanobeads. The magnetically labeled fraction is retained by the use of a magnetic separator. The untouched neutrophils are collected by decanting the liquid in a clean tube. These are the cells of interest; do not discard the liquid. Some of the downstream applications include functional assays, gene expression, phenotypic characterization, etc.
MojoSort™ reagents are also compatible with column-based cell separation systems available from other vendors. Optimized protocols for cell separation using columns from in-house testing are provided for each kit under the “Related Protocols” section, as well as representative data on the product webpage (where available). Data generated using column separators are indicated on the figure legend.
Due to the property of the beads, MojoSort™ reagents typically require dilution for optimal use on column separators. Where available, recommended dilution factors for each kit component based on in-house testing are provided under the “Application Notes” section of the webpage.
Kit Contents
- Kit Contents
-
For Cat# 480152:
- 1ml Biotin-Antibody Cocktail
- 1ml Streptavidin nanobeads
Product Details
- Verified Reactivity
- Human
- Formulation
- Phosphate buffer solution containing 0.09% sodium azide, pH 7.2.
- Preparation
-
The antibodies were purified by affinity chromatography, and conjugated with biotin under optimal conditions.
Streptavidin Nanobeads: Streptavidin-coated magnetic beads. - Storage & Handling
- All components should be stored undiluted between 2°C and 8°C.
- Application
-
Cell Separation (MojoSort™) - Quality tested
- Recommended Usage
-
10 µL of antibody cocktail for 1 mL of blood.
10 µL Streptavidin Nanobeads for 1 mL of blood. - Application Notes
-
This kit is designed for the isolation of untouched Human Neutrophils from whole blood.
Each lot has been individually optimized. Do not mix and match components from different lots or different kits.
Antibody or cocktail dilution to use in column: 1X
Nanobead dilution to use in columns: N/A
Antigen Details
- Cell Type
- Granulocytes
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Immunology
- Gene ID
- NA
- UniProt
- View information about Neutrophil on UniProt.org
Related FAQs
- Is there a way to detach your magnetic particles from the cell surface?
-
MojoSort™ Nanobeads: These magnetic particles cannot be removed from cells. This includes standalone Nanobeads and MojoSort kits using Nanobeads. We have found that cells are functional without the need to detach the magnetic Nanobeads.
MojoSort Microbeads: These magnetic particles may be released following the detailed product-specific protocol.
- What is the size of your magnetic particles?
-
MojoSort Nanobeads: the average diameter is approximately 130 nm.
MojoSort Microbeads: the average diameter is approximately 1 μm.
- Are MojoSort™ Nanobeads compatible with other commercially available magnetic separation systems?
-
MojoSort™ magnetic particles can be used with other commercially available magnetic separators, both free standing magnets and column-based systems. Because MojoSort™ protocols are optimized for the MojoSort™ separator, the protocols may need to be adjusted for other systems. Please contact BioLegend Technical Service for more product-specific information and guidance. We do not recommend using MojoSort™ particles for BD’s IMag™ or Life Technologies’ DynaMag™.
- What antibodies are present in the depletion cocktails provided for isolation kits?
-
Please contact our technical service team for further assistance.
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