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ELISA MAX™ Deluxe Set Mouse IL-1β

Pricing & Availability
Regulatory Status
RUO
Other Names
IL-1b ELISA kit
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Product Citations
publications
mouse il-1b_122109
  • mouse il-1b_122109
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432604 5 plates $370
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432615 10 Plates $586
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432616 20 Plates $938
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Description

ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Mouse IL-1β ELISA MAX™ Capture Antibody (200X)
  • Mouse IL-1β ELISA MAX™ Detection Antibody (200X)
  • Mouse IL-1β Standard
  • Avidin-HRP (1000X)
  • Substrate Solution D
  • Coating Buffer A (5X)
  • Assay Diluent A (5X)

Product Details

Verified Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application Notes

The assay kits will no longer include ELISA microwell plates. Recommended microwell plates can be purchased separately under Cat. No. 423501. Please contact your BioLegend sales representative for bulk pricing quotations.

BioLegend's mouse and human IL-1β ELISA kits use antibodies raised against the mature form of IL-1β. However, because pro-IL-1β contains the mature form, these antibodies likely recognize both the pro- and the mature forms of the protein. The human IL-1β ELISA kits have been shown to equally recognize both pro- and mature forms of IL-1β.

Application References

(PubMed link indicates BioLegend citation)
  1. O'Sullivan BJ, et al. 2006. J. Immunol. 176:7278.
  2. Huck O, et al. 2014. Innate Immun. 21:65. PubMed
  3. Alves BN, et al. 2014. J. Immunol. 192:3121. PubMed
  4. Haldar S, et al. 2015. J Biol Chem. 290:6574. PubMed
Product Citations
  1. Guo W, et al. 2021. Int J Biol Sci. 17:4271. PubMed
  2. Guo H, et al. 2020. Curr Protoc Immunol. 131:e107. PubMed
  3. Wang JJ, et al. 2022. J Biol Chem. 298:102017. PubMed
  4. Yao R, et al. 2022. Oxid Med Cell Longev. 2022:9469143. PubMed
  5. Yoo HJ, et al. 2022. Cell Rep. 41:111598. PubMed
  6. Zheng Y, et al. 2023. BMC Oral Health. 23:14. PubMed
  7. Zhao C, et al. 2023. NPJ Biofilms Microbiomes. 9:8. PubMed
  8. Hsu CG, et al. 2022. Cell Death Differ. 29:1790. PubMed
  9. Yan K, et al. 2022. Phytother Res. 36:2161. PubMed
  10. Dong X, et al. 2019. ACS Nano. 13:1272. PubMed
  11. Singh A, et al. 2023. Front Immunol. 14:1141712. PubMed
  12. Foster SL, et al. 2023. Cells. 12:. PubMed
  13. Liu X, et al. 2022. Biomedicines. 10:. PubMed
  14. Shih S, et al. 2016. PLoS One. 11: 0157647. PubMed
  15. Liu S, et al. 2022. Front Pharmacol. 13:847605. PubMed
  16. Piedra-Quintero ZL, et al. 2019. Front Immunol. 9:3118. PubMed
  17. Du L, et al. 2022. Aging (Albany NY). 14:6626. PubMed
  18. Schappe MS, et al. 2018. Immunity. 48:59. PubMed
  19. Gyorke CE, et al. 2020. J Immunol. 205:3037. PubMed
  20. Naji A, et al. 2016. Sci Rep. 6: 26162. PubMed
  21. Sun T, et al. 2018. Front Immunol. 9:1245. PubMed
  22. Yin Y, et al. 2022. Front Pharmacol. 13:882924. PubMed
  23. Zhang B, et al. 2021. Exp Ther Med. 22:832. PubMed
  24. Haldar S, et al. 2015. J Biol Chem. 290:6574. PubMed
  25. Liu X, et al. 2021. Int J Mol Sci. 22:. PubMed
  26. Shaker ME, et al. 2022. Saudi Pharm J. 30:291. PubMed
  27. Lin CC, et al. 2021. Iran J Basic Med Sci. 24:1146. PubMed
  28. Sousa AH, et al. 2021. Can J Physiol Pharmacol. 99:1324. PubMed
  29. Lian H, et al. 2021. Nat Microbiol. 6:658. PubMed
  30. Li Y, et al. 2021. Sci Transl Med. 13:. PubMed
  31. Huck O, et al. 2014. Innate Immunity. 21:65. PubMed
  32. Liu R, et al. 2018. Exp Ther Med. 15:3385. PubMed
  33. Zhou J, et al. 2022. Cancers (Basel). 14:. PubMed
  34. Liu TH, et al. 2018. Nutrients. 10:E472. PubMed
  35. Qi S, et al. 2022. Transl Stroke Res. :. PubMed
  36. Hsu CG, et al. 2021. J Immunol. 206:3010. PubMed
  37. Alves B, et al. 2014. J Immunol. 192:3121. PubMed
  38. Wilson JL, et al. 2020. Cell Rep. 30:1542. PubMed
  39. Swaroop S, et al. 2018. J Neuroinflammation. 15:3385. PubMed
  40. Zeng Q, et al. 2022. iScience. 25:105151. PubMed
  41. Tao X, et al. 2021. Front Physiol. 12:687046. PubMed
  42. Yang FM, et al. 2021. Proc Natl Acad Sci U S A. 118:. PubMed
  43. Zeis T, et al. 2015. Brain Behav Immun. 48: 313-325. PubMed
  44. Gu H, et al. 2021. Elife. 10:. PubMed
  45. Schaafsma W, et al. 2015. Brain Behav Immun. 48: 205-221. PubMed
  46. Wang Y, et al. 2021. PLoS One. 16:e0251578. PubMed
  47. Mantsounga CS, et al. 2022. Cell Rep. 38:110309. PubMed
  48. Kalia I, et al. 2022. Front Immunol. 12:699887. PubMed
  49. Healy A, et al. 2020. Arterioscler Thromb Vasc Biol. 40:714. PubMed
  50. Chen Y, et al. 2018. Exp Ther Med. 16:4322. PubMed
Sensitivity
16 pg/mL
Standard Range
31.3-2,000 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • PBS (Phosphate-Buffered Saline): 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add deionized water to 1 L; pH to 7.4, 0.2 µm filtered.
  • Wash Buffer (Phosphate-Buffered Saline (PBS) + 0.05% Tween-20, pH=7.4). BioLegend Cat. No. 421601 is recommended.
  • Wash bottle or automated microplate washer
  • Stop Solution (e.g. 2N H2SO4) Biolegend Cat. No. 423001 is recommended
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Plate Sealer
  • Absorbent paper

Antigen Details

Cell Sources
Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
16176 View all products for this Gene ID
UniProt
View information about IL-1beta on UniProt.org

Related FAQs

Do your IL-1β ELISA kits detect pro-IL-1β or the mature form?

The antibodies in our IL-1β kits were generated against the mature form of the protein. However, because the pro-form of the protein contains the mature form, the antibodies should detect both forms.

Do the ELISA MAX™ Deluxe Sets come with plates?

No, ELISA MAX™ Deluxe Sets do not come with plates. Coating Buffer and Assay Diluent (for blocking and dilutions) are included in these sets. Plates can be ordered separately (Cat No. 423501), and instructions for coating the plates are in the sets’ product manuals.

Go To Top Version: 6    Revision Date: 08/08/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

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