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ELISA MAX™ Standard Set Mouse IL-1β

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Regulatory Status
RUO
Other Names
IL-1b ELISA kit
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Product Citations
publications
mouse il-1b_122109
  • mouse il-1b_122109
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432601 5 plates $329
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Description

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ELISA Development Systems Engineered for Sensitive, Precise and Reliable Serum and Plasma Cytokine Quantitation

BioLegend's ELISA Max™ Sets provide all the time-savings, convenience and performance of a high-end, commercial ELISA kit for a fraction of the price! BioLegend's ELISA Max™ Sets, in two appealing formats, address the need for sensitivity, accuracy, dependability, and versatility of an ELISA development system, while providing maximum cost effectiveness, value, and ease of use.

Both the ELISA Max™ Set Standard and ELISA Max™ Set Deluxe are specifically engineered for accurate recovery and measurement of analytes in complex fluids, such as serum and plasma (as well as activated cell cultures) without any interference by confounding serum factors, such as heterophilic antibody or rheumatoid factor.

Product Details
Technical data sheet

Kit Contents

Kit Contents
  • Mouse IL-1β ELISA MAX™ Capture Antibody (200X)
  • Mouse IL-1β ELISA MAX™ Detection Antibody (200X)
  • Mouse IL-1β Standard
  • Avidin-HRP (1000X)

Product Details

Verified Reactivity
Mouse
Application

ELISA
 
As a part of BioLegend's efforts to go green, each new lot of the ELISA MAX™ sets will include a summarized version of its manual. Click here for more information.
Application Notes

BioLegend's mouse and human IL-1β ELISA kits use antibodies raised against the mature form of IL-1β. However, because pro-IL-1β contains the mature form, these antibodies likely recognize both the pro- and the mature forms of the protein. The human IL-1β ELISA kits have been shown to equally recognize both pro- and mature forms of IL-1β.

Application References

(PubMed link indicates BioLegend citation)
  1. O'Sullivan BJ, et al. 2006. J. Immunol. 176:7278.
  2. Murayama MA, et al. 2014. Biochem Biophys Res Commun. 443:42. PubMed
  3. Rapsinski GJ, et al. 2015. Infect Immun. 83:693. PubMed
Product Citations
  1. Chen Z, et al. 2022. Arthritis Rheumatol. 74:1544. PubMed
  2. Sun XY, et al. 2023. Signal Transduct Target Ther. 8:30. PubMed
  3. Samra YA, et al. 2020. J Biochem Mol Toxicol. 34:e22470. PubMed
  4. Zhao C, et al. 2022. Microbiol Spectr. 10:e0081122. PubMed
  5. Tang C, et al. 2023. Nat Commun. 14:1493. PubMed
  6. Sun Q, et al. 2023. Transl Neurosci. 14:20220273. PubMed
  7. Gawish R, et al. 2022. Elife. 11:. PubMed
  8. Santos I, et al. 2020. Proc Natl Acad Sci U S A. 117:12281. PubMed
  9. Poli V, et al. 2021. iScience. 24:103256. PubMed
  10. Theivanthiran B, et al. 2020. J Clin Invest. 130:2570. PubMed
  11. Guo Y, et al. 2020. J Clin Invest. 130:6301. PubMed
  12. Th?nert R, et al. 2017. Nat Commun. 8:14268. PubMed
  13. Li H, et al. 2021. Mol Med Rep. 1:23. PubMed
  14. Xu J, et al. 2020. J Am Soc Nephrol. 31:54:00. PubMed
  15. Li Y, et al. 2021. Cardiovasc Res. 890:117. PubMed
  16. Guo Y, et al. 2021. Front Cell Dev Biol. 9:743335. PubMed
  17. Li X, et al. 2019. Immunity. 50:591. PubMed
  18. Kolar SL, et al. 2019. JCI Insight. 4:5. PubMed
  19. Jung S, et al. 2021. Endocrinology. 162(3):. PubMed
  20. Li HY, et al. 2021. Cell Mol Gastroenterol Hepatol. 13:289. PubMed
  21. Xiang K, et al. 2022. Front Immunol. 13:814319. PubMed
  22. Chowdhury D, et al. 2021. Front Immunol. 12:755961. PubMed
  23. Andrade-Sousa A, et al. 2016. PLoS One. 11: 0163420. PubMed
  24. Murayama M, et al. 2014. Biochem Biophys Res Commun. 443:42. PubMed
  25. Marrocco A, et al. 2021. J Immunol. 207:1627. PubMed
  26. Zhuo X, et al. 2021. Biomed Rep. 15:64. PubMed
  27. Brown P, et al. 2015. PLoS One. 10:125126. PubMed
  28. Chen C, et al. 2017. Mol Med Rep. 15:2289. PubMed
  29. Li L, et al. 2022. Stem Cell Res Ther. 13:479. PubMed
  30. Rapsinski G, et al. 2015. Infect Immun . 83:693. PubMed
  31. Batista SJ, et al. 2020. Nat Commun. 3.01875. PubMed
  32. Segovia M, et al. 2019. Cancer Cell. 35:767. PubMed
  33. Tan EE, et al. 2020. J Clin Invest. 130:5817. PubMed
  34. Wong MY, et al. 2020. J Neuroinflammation. 0.841666667. PubMed
RRID
AB_2883996 (BioLegend Cat. No. 432601)
Standard Range
31.3-2,000 pg/mL
Materials Not Included
  • Microwell plates: 96-well Nunc MaxiSorp™ is recommended.
  • A microplate reader capable of measuring absorbance at 450 nm
  • Adjustable pipettes to measure volumes ranging from 2 µL to 1 mL
  • Deionized (DI) water
  • Coating Buffer: 8.4 g NaHCO3, 3.56 g Na2CO3, add DI H2O to 1.0 L, pH to 9.5 (BioLegend Cat. No. 421701 is recommended.)
  • Assay Diluent: 10% Fetal Bovine Serum or 1% BSA in Phosphate-Buffered Saline (PBS) (BioLegend Cat. No. 421203 is recommended.)
  • PBS: 8.0 g NaCl, 1.16 g Na2HPO4, 0.2 g KH2PO4, 0.2 g KCl, add DI water to 1.0 L, pH to 7.4
  • Wash Buffer: Phosphate-Buffered Saline (PBS) + 0.05% Tween-20 (BioLegend Cat. No. 421601 is recommended.)
  • Wash bottle or automated microplate washer
  • TMB Substrate Solution - BioLegend Cat. No. 421101 is recommended.
  • Stop Solution (2 N H2SO4)
  • Log-Log graph paper or software for data analysis
  • Tubes to prepare standard dilutions
  • Timer
  • Absorbent paper

Antigen Details

Cell Sources
Monocytes, tissue macrophages, Langerhan cells, dendritic cells, T lymphocytes, B lymphocytes, natural killer (NK) cells, large granular lymphocytes (LGL), vascular endothelium, smooth muscle, fibroblasts, thymic epithelia, astrocytes, microglia, glioma
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
Cytokines/Chemokines
Gene ID
16176 View all products for this Gene ID
UniProt
View information about IL-1beta on UniProt.org

Related FAQs

Do your IL-1β ELISA kits detect pro-IL-1β or the mature form?

The antibodies in our IL-1β kits were generated against the mature form of the protein. However, because the pro-form of the protein contains the mature form, the antibodies should detect both forms.

Go To Top Version: 4    Revision Date: 08/08/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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