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Myeloid Activation Analysis Cocktail

Pricing & Availability
Clone
S18012C; 7-239; L243;
Regulatory Status
RUO
Other Names
Myeloid Activation
Isotype
Mouse IgG1, κ/Mouse IgG2a, κ
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Product Citations
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Myeloid_Activation_Analysis_Cocktail_1_022025-2
Human peripheral blood leukocytes were stimulated with 1 µg/mL of IFN-γ to mimic a bacterial infection (right), stimulated with 1 µg/mL of IFN-α2 to mimic a viral infection (middle) or left unstimulated (left) for 20 hours at 37°C. Cells were then stained with Myeloid Activation Analysis Cocktail. CD64-positive cells are shown in the gated population.
  • Myeloid_Activation_Analysis_Cocktail_1_022025-2
    Human peripheral blood leukocytes were stimulated with 1 µg/mL of IFN-γ to mimic a bacterial infection (right), stimulated with 1 µg/mL of IFN-α2 to mimic a viral infection (middle) or left unstimulated (left) for 20 hours at 37°C. Cells were then stained with Myeloid Activation Analysis Cocktail. CD64-positive cells are shown in the gated population.
  • Myeloid_Activation_Analysis_Cocktail_2_022025-2
    Human peripheral blood leukocytes were stimulated with 1 µg/mL of IFN-γ to mimic a bacterial infection (right), stimulated with 1 µg/mL of IFN-α2 to mimic a viral infection (middle) or left unstimulated (left) for 20 hours at 37°C. Cells were then stained with Myeloid Activation Analysis Cocktail. CD169-positive cells are shown in the gated population.
  • Myeloid_Activation_Analysis_Cocktail_3_022025-2
    Human peripheral blood leukocytes were stimulated with 1 µg/mL of IFN-γ to mimic a bacterial infection (right), stimulated with 1 µg/mL of IFN-α2 to mimic a viral infection (middle) or left unstimulated (left) for 20 hours at 37°C. Cells were then stained with Myeloid Activation Analysis Cocktail. HLA-DR-positive cells are shown in the gated population.
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391506 200 tests $890
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Description

The Myeloid Activation Analysis Cocktail facilitates rapid discrimination between viral and bacterial infections in a single simple test. This cocktail specifically helps to identify increased expression of CD169 on monocytes during viral infection and surface expression of CD64 on neutrophils in response to bacterial pathogens. It is optimized for use with a simplified No Wash staining protocol and analysis without the need for compensation.

The Myeloid Activation Analysis Cocktail contains:
PE anti-human CD169 (Sialoadhesin, Siglec-1) (clone 7-239)
APC anti-human CD64 (clone S18012C)
Spark Violet™ 423 anti-human HLA-DR (clone L243)

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Host Species
Mouse
Formulation
This cocktail contains PE, APC and Spark Violet™ 423 conjugated antibodies in an optimal concentration for flow cytometric analysis.
Preparation
All antibodies were purified by affinity chromatography and conjugated under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of the antibody cocktail is quality control tested. For flow cytometric staining, the suggested use of this reagent is 5 µL per 50 µL volume of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Protocol - Whole Blood Lyse/No Wash Procedure:

  1. Prepare 1X RBC Lysis/Fixation solution by diluting 1 part of BioLegend RBC Lysis/Fixation solution (Cat. No. 422401) with 9 parts room temperature deionized water.
  2. Add 5 µL of the Myeloid Activation Analysis Cocktail to tube/well.
  3. Add 45 µL of Cell Staining Buffer (Cat. No. 420201) or equivalent.
  4. Mix in 50 µL of whole blood.
  5. Incubate at room temperature, in the dark, for 20 minutes.
  6. Add 0.45 mL of 1X RBC Lysis/Fixation solution, gently mixing each tube immediately.
  7. Incubate at room temperature, in the dark, for 15 minutes.
  8.  Analyze samples by flow cytometry.

Antigen Details

Distribution

Myeloid cells

Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Innate Immune Signaling
Gene ID
2209 View all products for this Gene ID 6614 View all products for this Gene ID 3122 View all products for this Gene ID 3123 View all products for this Gene ID

Related FAQs

There are no FAQs for this product.
Go To Top Version: 1    Revision Date: 02/20/2025

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

BioLegend, the BioLegend logo, and all other trademarks are property of BioLegend, Inc. or their respective owners, and all rights are reserved.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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