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- Clone
- W18195C (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
- Isotype
- Rat IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
-
Kasumi-3 cells (human acute myeloblastic leukemia cell line) were stained with anti-human CD117(c-kit) (clone W18195C) Pacific Blue (filled histogram) or rat IgG2a κ Pacific Blue isotype control (open histogram).
| Cat # | Size | Price | Quantity Check Availability | Save | ||
|---|---|---|---|---|---|---|
| 375303 | 25 tests | $159 | ||||
| 375304 | 100 tests | $347 | ||||
CD117 is a 145 kD protein tyrosine kinase also known as c-kit. It is a receptor for stem cell factor or c-kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Human CD117 transfectant
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Clone W18195C antibody does not block the binding of clones 104D2 or S18022G to the target. Clone W18195C antibody does not bind to the target after cells are fixed by 4% PFA.
- RRID
-
AB_2890813 (BioLegend Cat. No. 375303)
AB_2890813 (BioLegend Cat. No. 375304)
Antigen Details
- Structure
- Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
- Distribution
-
Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)
- Function
- Receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
- Ligand/Receptor
- Stem cell factor (c-kit ligand)
- Cell Type
- Embryonic Stem Cells, Hematopoietic stem and progenitors, Leukemia, Mast cells, Mesenchymal Stem Cells
- Biology Area
- Immunology, Stem Cells
- Molecular Family
- CD Molecules
- Antigen References
-
1. Giebel LB, et al. 1992. Oncogene. 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736. - Gene ID
- 3815 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
Related Pages & Pathways
Pathways
Related FAQs
Other Formats
View All CD117 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD117 (c-kit) | W18195C | FC |
| PE/Cyanine7 anti-human CD117 (c-kit) Antibody | W18195C | FC |
| PE anti-human CD117 (c-kit) Antibody | W18195C | FC |
| APC anti-human CD117 (c-kit) | W18195C | FC |
| Pacific Blue™ anti-human CD117 (c-kit) | W18195C | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-human CD117 (c-kit)
Kasumi-3 cells (an acute myeloblastic leukemia cell line) we... -
PE/Cyanine7 anti-human CD117 (c-kit) Antibody
Kasumi-3 cells (human acute myeloblastic leukemia cell line)... -
PE anti-human CD117 (c-kit) Antibody
Kasumi-3 cells (human acute myeloblastic leukemia cell line)... -
APC anti-human CD117 (c-kit)
Kasumi-3 cells (human acute myeloblastic leukemia cell line)... -
Pacific Blue™ anti-human CD117 (c-kit)
Kasumi-3 cells (human acute myeloblastic leukemia cell line)...
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