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Pacific Blue™ anti-human CD14 Antibody

Pricing & Availability
Clone
HCD14 (See other available formats)
Regulatory Status
RUO
Other Names
LPS receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
HCD14_PB_040711
Human peripheral blood monocytes were stained with CD14 (HCD14) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram).
  • HCD14_PB_040711
    Human peripheral blood monocytes were stained with CD14 (HCD14) Pacific Blue™ (filled histogram) or mouse IgG1, κ Pacific Blue™ isotype control (open histogram).
Compare all formats See Pacific Blue™ spectral data
Cat # Size Price Quantity Check Availability Save
325615 25 µg $112
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325616 100 µg $265
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein also known as LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokines expression in monocytes and neutrophils.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 2.0 µg per 106 cells in 100 µl volume or 100 µl of whole blood. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunofluorescence microscopy. This clone was tested in-house and does not work on formalin fixed paraffin-embedded (FFPE) tissue.

Application References

(PubMed link indicates BioLegend citation)
  1. McMichael A, et al. 1987. Leucocyte Typing III. Oxford University Press. New York.
  2. Knapp W, et al. Eds. 1989. Leucocyte Typing IV. Oxford University Press. New York.
  3. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
Product Citations
  1. Ghassemi S, et al. 2022. Nat Biomed Eng. 6:118. PubMed
  2. Johnson L, Olson B, and McNeel D. 2017. J Immunother Cancer. 10.1186/s40425-017-0260-3. PubMed
  3. Liisborg C, et al. 2022. Acta Ophthalmol. :3. PubMed
  4. Zheng Y, et al. 2022. Proc Natl Acad Sci U S A. 119:e2121077119. PubMed
  5. Liu Y, et al. 2023. Nat Commun. 14:2179. PubMed
  6. Johnson LE, et al. 2017. J Immunother Cancer. 5:56. PubMed
  7. Davis Z, et al. 2011. J Vis Exp. 49: 2668. PubMed
  8. Dockree T, et al. 2016. Immunol Cell Biol. 95:68-76. PubMed
  9. Pachnio A, et al. 2016. PLoS Pathog. 12: 1005832. PubMed
  10. Paubelle E, et al. 2020. Cell Reports. 30(3):739-754.e4.. PubMed
  11. Crespo ÂC, et al. 2020. Cell. 182(5):1125-1139. PubMed
  12. Schmidleithner L et al. 2019. Immunity. 50(5):1232-1248 . PubMed
  13. Queckborner S, et al. 2020. Stem Cell Res Ther. 11:15. PubMed
  14. Demirel &, et al. 2007. J Biol Chem. 282:37836. PubMed
  15. Liisborg C, et al. 2022. PLoS One. 17:e0269960. PubMed
  16. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  17. Tu HA, et al. 2020. Cell Rep Med. 1:100155. PubMed
  18. Séguier S, et al. 2013. PLoS One. 8:70937. PubMed
  19. Li G, et al. 2019. J Virol. 93:e00233-19. PubMed
  20. Lu J, et al. 2021. Front Immunol. 12:732612. PubMed
  21. Barman S, et al. 2016. Int Immunol. 28: 533 - 545. PubMed
  22. Glaser K, et al. 2016. PLoS One. 11: 0146898. PubMed
  23. Roerden M, et al. 2021. Cancers. 13(11):. PubMed
  24. Collison J, et al. 2015. J Immunol. 195: 1162-1170. PubMed
  25. Serr I, et al. 2016. Nat Commun. 7:10991. PubMed
  26. Yang C, et al. 2019. Nat Commun. 10:3931. PubMed
RRID
AB_830689 (BioLegend Cat. No. 325615)
AB_830689 (BioLegend Cat. No. 325616)

Antigen Details

Structure
GPI-linked membrane glycoprotein, 53-55 kD
Distribution

Monocytes, macrophages, granulocytes (low)

Function
LPS receptor, clearance of Gram-negative pathogens
Ligand/Receptor
LPS
Cell Type
Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules
Antigen References

1. Stocks S, et al. 1990. Biochem. J. 268:275.
2. Wright S, et al. 1990. Science 249:1434.

Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

Related FAQs

There are no FAQs for this product.
Go To Top Version: 2    Revision Date: 07/13/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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