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Pacific Blue™ anti-mouse Ly-6A/E (Sca-1) Antibody

Pricing & Availability
Clone
E13-161.7 (See other available formats)
Regulatory Status
RUO
Other Names
Sca-1
Isotype
Rat IgG2a, κ
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Product Citations
publications
E13-161dot7_Blue_091207
C57BL/6 mouse splenocytes stained with Pacific Blue™ E13-161.7
  • E13-161dot7_Blue_091207
    C57BL/6 mouse splenocytes stained with Pacific Blue™ E13-161.7
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122519 25 µg $101
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122520 100 µg $229
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Description

Ly-6A/E is an 18 kD member of the Ly-6 multigene family also known as Sca-1. Ly6A/E is a glycosylphosphatidylinositol (GPI)-linked protein expressed on hematopoietic stem cells. In mice expressing the Ly-6.2 haplotype (e.g., AKR, C57BL, C57BR, DBA/2, SJL, SWR, and 129), Ly-6A/E is also expressed on peripheral B lymphocytes and thymic and peripheral T lymphocytes. Strains expressing the Ly-6.1 haplotype (e.g., BALB/c, CBA, C3H/He, DBA/1, and NZB) have low Ly-6A/E expression on resting peripheral lymphocytes. The expression of Ly-6A/E on lymphocytes is upregulated upon activation from both Ly6.1 and Ly6.2 haplotype mice. Ly-6A/E is thought to be involved in the regulation of both T and B cell responses. The E13-161.7 antibody has been reported to be useful for immunohistochemical staining of frozen sections, immunoprecipitation, flow cytometry, and complement-mediated cytotoxicity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mouse pre-T cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with Pacific Blue™ under optimal conditions.
Concentration
0.5 mg/ml
Storage & Handling
The Ly-6A/E antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. The suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is highly recommended that the reagent be titrated for optimal performance for each application.

* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.


Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.

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Excitation Laser
Violet Laser (405 nm)
Application Notes

Additional reported applications (for the relevant formats) include: complement-mediated cytoxicity1, immunohistochemical staining of frozen sections2 and Bouin-fixed, paraffin-embedded samples4, immunofluorescence microscopy4, and immunoprecipitation3.

The two Sca-1 recognizing clones D7 and E13-161.7 have been shown to bind distinct epitopes due to the inability of D7 to block the binding of E13-161.7.7

Application References

(PubMed link indicates BioLegend citation)
  1. Aihara Y, et al. 1986. Eur. J. Immunol. 16:1391. (CMCD)
  2. Spangrude GJ, et al. 1988. J. Immunol. 141:3697. (IHC)
  3. van de Rijin M, et al. 1989. P. Natl. Acad. Sci. USA 86:4634. (IP)
  4. van Bragt MPA, et al. 2005. Biol. Reprod. 73:634. (IHC, IF)
  5. Rosas M, et al. 2010. J. Leukoc. Biol. 88:169. PubMed
  6. Felthause O, et al. 2010. Neurosci Lett. 471:179. PubMed
  7. Bamezai A and Rock KL. 1995. Proc. Natl. Acad. Sci. USA 92:4294.
  8. Artinger EL, et al. 2013. PNAS. 110:12000. PubMed
Product Citations
  1. Hu W, et al. 2015. Blood. 125:2206. PubMed
  2. Barrett N, et al. 2016. Cell Rep. 16: 1039-1054. PubMed
  3. Lawson H, et al. 2021. Stem Cell Reports. 16:2784. PubMed
  4. Chen J, et al. 2022. STAR Protoc. 3:101849. PubMed
  5. Yuan O, et al. 2022. Sci Adv. 8:eabm9987. PubMed
  6. Rundberg Nilsson A, et al. 2023. iScience. 26:106341. PubMed
  7. Herrejon Chavez F, et al. 2023. Nat Commun. 14:2290. PubMed
  8. Symeonidou V, et al. 2021. Cell Rep. 37:109900. PubMed
  9. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  10. Fossati V, et al. 2010. J Immunol. 184:1251. PubMed
  11. Karra L, et al. 2020. Oncogene. 6920:39. PubMed
  12. Wong J, et al. 2015. Elife. 3: 07839. PubMed
  13. Grassinger J, et al. 2009. Blood. 114:49:00. PubMed
  14. Liu Y, et al. 2021. Haematologica. Online ahead of print. PubMed
  15. Nguyen DTT, et al. 2020. Nat Commun. 11:2026. PubMed
  16. Eisele AS, et al. 2022. Elife. 11:. PubMed
  17. Lopez DA, et al. 2022. Cell Rep. 41:111677. PubMed
  18. Sala D, et al. 2019. Nat Commun. 10:1796. PubMed
  19. Courtney AH et al. 2017. Molecular cell. 67(3):498-511 . PubMed
  20. Yankova E, et al. 2021. Nature. 593:597. PubMed
  21. Booth CAG, et al. 2018. Cancer Cell. 33:274. PubMed
  22. Cheng Y, et al. 2019. Cell Rep. 28:1703. PubMed
  23. Zhang H, et al. 2019. Mol Cell. 76:110. PubMed
  24. Oben K, et al. 2017. PLoS One. 10.1371/journal.pone.0169767. PubMed
  25. Tzelepis K, et al. 2018. Nat Commun. 9:5378. PubMed
  26. Gerdes P, et al. 2022. Nat Commun. 13:7470. PubMed
  27. Ortega-Molina A, et al. 2021. Cell Reports. 36(2):109372. PubMed
  28. Yue F, et al. 2022. Cell Rep. 38:110267. PubMed
  29. Lee SG, et al. 2020. J Nucl Med. . PubMed
  30. Spevak CC, et al. 2020. Cell Stem Cell. 26(5):755-765. PubMed
  31. Säwen P et al. 2018. eLife. 7 pii: e41258. PubMed
  32. Park SM et al. 2018. Cell stem cell. 24(1):153-165 . PubMed
  33. Gozdecka M, et al. 2018. Nat Genet. 50:883. PubMed
  34. Ellis S, et al. 2011. Blood. 118:1516. PubMed
  35. Jee D et al. 2018. Molecular cell. 69(2):265-278 . PubMed
RRID
AB_2143237 (BioLegend Cat. No. 122519)
AB_2143237 (BioLegend Cat. No. 122520)

Antigen Details

Structure
Ly-6 multigene family, 18 kD
Distribution

Hematopoietic stem cells, activated T cells and B cells, subset of resting B cells and T cells

Function
Regulates B and T cell responses
Cell Type
B cells, Hematopoietic stem and progenitors, Mesenchymal Stem Cells, T cells
Biology Area
Immunology, Stem Cells
Antigen References

1. Rock KL, et al. 1989. Immunol. Rev. 111:195.
2. Morrison SJ, et al. 1994. Immunity 1:661.
3. Spangrude GJ, et al. 1988. J. Immunol. 141:3697.
4. Malek T, et al. 1986. J. Exp. Med. 164:709.

Gene ID
110454 View all products for this Gene ID
UniProt
View information about Ly-6A/E on UniProt.org
Go To Top Version: 3    Revision Date: 07/11/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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