- Clone
- MR9-4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Vβ5 T-cell receptor, T cell receptor beta 5
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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139515 | 25 µg | $101 | ||||
139516 | 100 µg | $229 |
Vβ5.1 and 5.2 T cell receptor (TCR Vβ5.1, 5.2) are variants of TCR β chain that, along with TCR α chain, forms the TCR heterodimer. In association with the CD3 complex, TCR α/β is responsible for antigen recognition in the MHC-Peptide complex and the initiation of T cell-mediated immune responses.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Murine T cell hybridoma 2HB51.8
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody was purified by affinity chromatography and conjugated with Pacific Blue™ under optimal conditions. The solution is free of unconjugated Pacific Blue™.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
* Pacific Blue™ has a maximum emission of 455 nm when it is excited at 405 nm. Prior to using Pacific Blue™ conjugate for flow cytometric analysis, please verify your flow cytometer's capability of exciting and detecting the fluorochrome.
Alexa Fluor® and Pacific Blue™ are trademarks of Life Technologies Corporation.
View full statement regarding label licenses - Excitation Laser
-
Violet Laser (405 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: Induction of proliferation of Vß5.1+ and Vß5.2+ T cells2, 3 and in vivo depletion of Vß5+ T cells4.
-
Application References
(PubMed link indicates BioLegend citation) -
- Kanagawa O, et al. 1991. J. Immunol. 147:1307. (FC)
- Kanagawa O, et al. 1992. J. Immunol. 149:9. (Activ)
- Woodland DL, et al. 1993. J. Exp. Med. 177:433. (Activ)
- Gelber C, et al. 1992. Cancer Res. 52:6507. (Deplete)
- Product Citations
-
- RRID
-
AB_2750134 (BioLegend Cat. No. 139515)
AB_2750134 (BioLegend Cat. No. 139516)
Antigen Details
- Structure
- Member of the immunoglobulin superfamily, Vβ5.1and 5.2 are variants of the TCR β chain which associates with the TCR α chain to form the TCR heterodimer.
- Distribution
-
Expressed on a subset of TCR αβ+ T cells.
- Function
- Recognition of peptides presented by the MHC molecules, responsible for T cell mediated immune responses.
- Ligand/Receptor
- MHC-Peptide complex
- Cell Type
- T cells
- Biology Area
- Adaptive Immunity, Immunology
- Molecular Family
- TCRs
- Antigen References
-
1. Marrack P, et al. 2008. Annu. Rev. Immunol. 26:171.
2. Sim GK and Augustin AA. 1985. Cell 42:89.
3. Mami-Chouaib F, et al. 2002. Immunol. Rev. 188:114. - Gene ID
- 21577 View all products for this Gene ID
- UniProt
- View information about TCR Vbeta5.1 5.2 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
Other Formats
View All TCR Vβ5.1, 5.2 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
APC anti-mouse TCR Vβ5.1, 5.2 | MR9-4 | FC |
PE anti-mouse TCR Vβ5.1, 5.2 | MR9-4 | FC |
PE/Cyanine7 anti-mouse TCR Vβ5.1, 5.2 | MR9-4 | FC |
PerCP/Cyanine5.5 anti-mouse TCR Vβ5.1, 5.2 | MR9-4 | FC |
Pacific Blue™ anti-mouse TCR Vβ5.1, 5.2 | MR9-4 | FC |
FITC anti-mouse TCR Vβ5.1, 5.2 | MR9-4 | FC |
APC/Fire™ 750 anti-mouse TCR Vβ5.1, 5.2 | MR9-4 | FC |
TotalSeq™-A0354 anti-mouse TCR Vβ5.1, 5.2 | MR9-4 | PG |
TotalSeq™-C0354 anti-mouse TCR Vβ5.1, 5.2 | MR9-4 | PG |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-mouse TCR Vβ5.1, 5.2
C57BL/6 splenocytes were stained with CD3-PE and TCR Vβ5.1, ... -
PE anti-mouse TCR Vβ5.1, 5.2
C57BL/6 splenocytes stained with CD3e (145-2C11) APC and MR9... C57BL/6 splenocytes stained with CD3e (145-2C11) APC and mou... -
PE/Cyanine7 anti-mouse TCR Vβ5.1, 5.2
C57BL/6 splenocytes were stained with CD3 APC and TCR Vβ5.1,... -
PerCP/Cyanine5.5 anti-mouse TCR Vβ5.1, 5.2
C57BL/6 splenocytes were stained with CD3e APC and TCR Vß5.1... -
Pacific Blue™ anti-mouse TCR Vβ5.1, 5.2
C57BL/6 splenocytes were stained with CD3ε PE and TCR Vβ5.1,... -
FITC anti-mouse TCR Vβ5.1, 5.2
C57BL/6 splenocytes were stained with CD3ε PE and TCR Vβ5.1,... -
APC/Fire™ 750 anti-mouse TCR Vβ5.1, 5.2
C57BL/6 splenocytes were stained with CD3ε PE and TCR Vβ5.1,... -
TotalSeq™-A0354 anti-mouse TCR Vβ5.1, 5.2
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TotalSeq™-C0354 anti-mouse TCR Vβ5.1, 5.2
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