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PE anti-AIM2 Antibody

Pricing & Availability
Clone
3B10 (See other available formats)
Regulatory Status
RUO
Other Names
Absent in melanoma 2 (AIM2), PYHIN4, interferon-inducible protein AIM2
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
3B10_PE_AIM2_Antibody_ICFC_080815
THP-1 cells (open histogram) and THP-1 cells stimulated with IFN-γ for 16 hours (filled histogram) were treated with the True-Nuclear™ Transcription Factor Buffer Set, and then were stained with AIM2 (clone 3B10) PE.
  • 3B10_PE_AIM2_Antibody_ICFC_080815
    THP-1 cells (open histogram) and THP-1 cells stimulated with IFN-γ for 16 hours (filled histogram) were treated with the True-Nuclear™ Transcription Factor Buffer Set, and then were stained with AIM2 (clone 3B10) PE.
See PE spectral data
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652803 25 tests $159
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652804 100 tests $360
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Description

AIM2 is one of the human IFN-inducible proteins, encoding a 39 kD protein. It contains a 200 amino acid repeat, which is a signature of the HIN-200 family (hematopoietic IFN-inducible nuclear proteins). Expression of AIM2 can inhibit cell growth and tumor formation, possibly through inducing apoptosis and suppressing nuclear factor-κ B transcriptional activity and desensitizing tumor necrosis factor-alpha-mediated nuclear factor-κ B activation. Furthermore, the gene for AIM2 shows a high frequency of mutations that are associated with microsatellite unstable colorectal cancers. AIM2 plays a critical role in the activation of caspase-1, the protease responsible for the processing of pro-inflammatory cytokines IL-1β and IL-18. AIM2 is also essential for mediating inflammatory reactions triggered by cytoplasmic DNA. AIM2 is an important sensor for different pathogens through the binding of cytoplasmic double-stranded DNA through HIN-200 domain and formation of inflammatsomes including ASC/AIM2. This complex then triggers caspase-1 activation. Recent studies also show that AIM2/ASC complex acts as a novel caspase-8 activation platform.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant fragment, corresponding to amino acids 1-186 of human AIM2 with a GST tag.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Western blotting1, immunofluorescence staining3 and immunoprecipitation2.

NOTE: For flow cytometric staining with this clone, True-Nuclear™ Transcription Factor Buffer Set (Cat. No. 424401) offers improved staining and is highly recommended.

Application References

(PubMed link indicates BioLegend citation)
  1. Pierini R, et al. 2012. Cell Death Differ. 19:1709. (WB)
  2. Fernandez-Alnemri T, et al. 2009. Nature 7237:509. (IP)
  3. Sagulenko V, et al. 2013. Cell Death Differ. 9:1149. (IF)
Product Citations
  1. Svensson A, et al. 2017. PLoS One.. 10.1371/journal.pone.0183268. PubMed
RRID
AB_2566000 (BioLegend Cat. No. 652803)
AB_2566000 (BioLegend Cat. No. 652804)

Antigen Details

Structure
343 amino acids with predicted molecular weight of 39 kD.
Distribution

Cytosol.

Function
Tumor suppressor which may act by repressing NF-κ B transcriptional activity.
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Immunology, Innate Immunity
Antigen References

1. DeYoung KL, et al. 1997. Oncogene 15:453.
2. Choubey D, et al. 2000. FEBS Lett. 474:38.
3. Chen IF, et al. 2006. Mol. Cancer Ther. 5:1.
4. Roberts TL, et al. 2009. Science 323:1057.
5. Jones JW, et al. 2010. Proc. Natl. Acad. Sci. USA 107:9771.
6. Fernandes-Alnemri T, et al. 2010. Nat. Immunol. 11:385.
7. Schroder K and Tschopp J. 2010. Cell 140:821.
8. Jin T, et al. 2012. Immunity 36:561.

Gene ID
9447 View all products for this Gene ID
UniProt
View information about AIM2 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08/21/2015

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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