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PE anti-ATM Phospho (Ser1981) Antibody

Pricing & Availability
Clone
10H11.E12 (See other available formats)
Regulatory Status
RUO
Other Names
Ataxia telangiectasia mutated (ATM) kinase, serine-protein kinase, AT mutated
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
10H1.1E12_PE_ATM_Antibody_FC_102813.jpg
Etoposide-treated, HeLa cells were treated with FOXP3 Fix/Perm Buffer Set and then intracellularly stained with anti-human/mouse ATM Phospho (Ser1981) (clone 10H11.E12) PE (filled histogram). Open histogram represents the staining with untreated HeLa cells.
  • 10H1.1E12_PE_ATM_Antibody_FC_102813.jpg
    Etoposide-treated, HeLa cells were treated with FOXP3 Fix/Perm Buffer Set and then intracellularly stained with anti-human/mouse ATM Phospho (Ser1981) (clone 10H11.E12) PE (filled histogram). Open histogram represents the staining with untreated HeLa cells.
See PE spectral data
Cat # Size Price Quantity Check Availability Save
651203 25 µg $177
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651204 100 µg $372
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Description

Ataxia telangiectasia mutated kinase (ATM) is a serine/threonine kinase that regulates cell cycle checkpoints and DNA repair. ATM is held inactive in unirradiated cells as a dimer or higher-order multimer. Cellular irradiation induces rapid intermolecular autophosphorylation of serine 1981 that causes dimer dissociation and initiates cellular ATM kinase activity. ATM kinase regulates a number of proteins involved in cell cycle checkpoint control, apoptosis, and DNA repair, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. Mutations in the corresponding ATM gene result in ataxia telangiectasia (AT), an autosomal recessive disease characterized by uncoordinated muscle movement and neurodegeneration. Cells from AT patients display defective DNA damage-induced checkpoint activation, sensitivity to radiation, and a higher frequency of chromosome breakage.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KLH conjugated synthetic peptide SLAFEEGSpQSTTISS
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Patent No. USA: 7,108,992

Product Citations
  1. Alissafi T, et al. 2020. Cell Metabolism. 32(4):591-604.e7. PubMed
  2. Ryl T et al. 2017. Cell systems. 5(3):237-250 . PubMed
  3. Welch G, et al. 2022. Sci Adv. 8:eabo4662. PubMed
  4. Yoon Y, et al. 2016. Stem Cell Reports. 7:840-853. PubMed
  5. Barili V, et al. 2020. Nat Commun. 0.877777778. PubMed
  6. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
RRID
AB_2562654 (BioLegend Cat. No. 651203)
AB_2562654 (BioLegend Cat. No. 651204)

Antigen Details

Structure
3056 amino acids, 350 kD.
Distribution

Primarily nuclear, also found in endocytic vesicles.

Function
Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis, and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor.
Biology Area
Cell Biology, Chromatin Remodeling/Epigenetics, Transcription Factors, Ubiquitin/Protein Degradation
Molecular Family
Phospho-Proteins
Antigen References

1. Lee JH and Paull TT 2007. Oncogene 26:7741.
2. Bakkenist CJ and Kastan MB 2003. Nature 421:499.
3. McConville CM, et al. 1996. Am. J. Hum. Genet. 59:320.
4. Tang X, et al. 2008. Mol. Cell Biol. 28:2559.
 

Gene ID
472 View all products for this Gene ID
UniProt
View information about ATM on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/13/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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