- Clone
- E23.1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- CCNA2, cyclin A2, CCN1, CCNA
- Isotype
- Mouse IgG2a, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
644003 | 25 tests | $153 | ||||
644004 | 100 tests | $335 |
Cyclins regulate the activity of the Cyclin-dependent protein kinases. Cyclin A is involved in the regulation of the cell cycle and seems to be essiential for S phase and M phase. Studies have shown that overexpression of cyclin A can accelerate the G1 to S phase. Cyclin A is a marker for actively proliferating cells and for cells in S phase.
Product DetailsProduct Details
- Verified Reactivity
- Human, Mouse
- Reported Reactivity
- Cow
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Cyclin A fusion protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Antibody may perform better for ICFC if cells sit in 70% ethanol or True Phos™ perm Buffer for 24 hours – 72 hours at -20° C
- RRID
-
AB_2715836 (BioLegend Cat. No. 644003)
AB_2715836 (BioLegend Cat. No. 644004)
Antigen Details
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication
- Antigen References
-
1. Pagano M et al. 1992. EMBO J 11:961
2. Geley S et al. 2001 J Cell Biol. 153:137
3. Resinitzky D et al. 1995. Mol Cell Biol 15:4347
4. Furuno N et al. 1999. J Cell Biol. 147:295 - Gene ID
- 890 View all products for this Gene ID
- UniProt
- View information about Cyclin A on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All Cyclin A Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-Cyclin A | E23.1 | WB,ICFC |
PE anti-Cyclin A | E23.1 | ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-Cyclin A
Total cell lysates (15 µg total protein) from the indicated ... NIH/3T3 cells were fixed with Fixation Buffer, permeabilized... Jurkat cells were fixed and permeabilized with cold 70% etha... -
PE anti-Cyclin A
NIH/3T3 cells were fixed with Fixation Buffer, permeabilized... Jurkat cells were fixed and permiabilized with cold 70% etha...
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