PE anti-HDAC1 Phospho Ser406 Antibody, HDAC1, BT-15

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Clone: BT-15 (See other available formats)
Regulatory Status: RUO
Other Names: Histone Deactylase 1, RPD3L1
Isotype: Mouse IgG2b, κ
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Product Citations: publications

BT-15_PE_HDAC1-phospho_Antibody_1_012219 — Untreated HeLa cells (left) and HeLa cells treated with Nocodazole for 24 hours (right), were fixed, permeabilized with True-Phos™ Perm Buffer, and then stained with anti-HDAC1 Phospho (Ser406) Antibody (clone BT-15) PE and DAPI.

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611003

25 tests

$153

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611004

100 tests

$325

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Description

Histone Deacetylase 1 (HDAC1) plays a critical role in various cellular processes, including cell cycle progression, proliferation, and differentiation. The enzyme functions by removing acetyl moieties from histone targets, resulting in histone compaction and alterations in nucleosomal positioning. Aurora kinases phosphorylate HDAC1 at Ser406 during prophase, immediately after cells begin mitosis, resulting in reduced deacetylase activity of HDAC1. This modification plays an essential role in regulating cell cycle progression, as well as controlling the expression of genes involved in central nervous system development.

Product Details

Technical data sheet

Product Details

Verified Reactivity: Human, Zebrafish
Antibody Type: Monoclonal
Host Species: Mouse
Immunogen: Synthetic peptide corresponding to human HDAC1 phosphorylated at Serine 406
Formulation: Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation: The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration: Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling: The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application: ICFC - Quality tested
Recommended Usage: Each lot of this antibody is quality control tested by intracellular flow cytometry using our True-Phos™ Perm Buffer in Cell Suspensions Protocol. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
Excitation Laser: Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes: When tested for western blot, this clone produced a band that showed a ~1-3 kD mass shift compared to a pan HDAC1 antibody. This observation is consistent with a previous study of the HDAC1 Phospho (Ser406) site.

This clone recognizes zebrafish HDAC1 phosphorylated at Ser406².

Clone BT-15 cross-reacts to Zebrafish²
RRID: AB_2801113 (BioLegend Cat. No. 611003)
AB_2801113 (BioLegend Cat. No. 611004)

Antigen Details

Structure: HDAC1 is a 482 amino acid protein with a predicted molecular weight of 55 kD.
Distribution: Nucleus/Ubiquitously expressed
Function: Phosphorylation of HDAC1 Ser406 results in reduced deactylase activity of the enzyme.
Biology Area: Cell Biology, Cell Cycle/DNA Replication, Chromatin Remodeling/Epigenetics
Molecular Family: Nuclear Markers, Phospho-Proteins
Antigen References: Segre CV, et al, 2016. mAbs. 8: 37-42.

Loponte S, et al, 2016. Sci Rep, 6: 30213.
Gene ID: 3065 View all products for this Gene ID
UniProt: View information about HDAC1 Phospho Ser406 on UniProt.org

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What type of PE do you use in your conjugates?: We use R-PE in our conjugates.

Other Formats

View All HDAC1 Phospho (Ser406) Reagents Request Custom Conjugation

Description	Clone	Applications
PE anti-HDAC1 Phospho (Ser406)	BT-15	ICFC
Purified anti-HDAC1 Phospho (Ser406)	BT-15	WB,ICFC
Alexa Fluor® 488 anti-HDAC1 Phospho (Ser406)	BT-15	ICFC
PE/Cyanine5 anti-HDAC1 Phospho (Ser406)	BT-15	ICFC
Alexa Fluor® 647 anti-HDAC1 Phospho (Ser406)	BT-15	ICFC

Certificate of Analysis

Go To Top Version: 1 Revision Date: 01/22/2019

For Research Use Only. Not for diagnostic or therapeutic use.

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

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