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PE anti-human CD14 Antibody

Pricing & Availability
Clone
63D3 (See other available formats)
Regulatory Status
RUO
Other Names
Monocyte differentiation antigen CD14, myeloid cell-specific leucine-rich glycoprotein, LPS receptor
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
63D3_PE_CD14_Antibody_FC_061915
Human peripheral blood monocytes were stained with CD14 (clone 63D3) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
  • 63D3_PE_CD14_Antibody_FC_061915
    Human peripheral blood monocytes were stained with CD14 (clone 63D3) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).
Compare all formats See PE spectral data
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367103 25 tests $110
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367104 100 tests $250
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Description

CD14 is a 53-55 kD glycosylphosphatidylinositol (GPI)-linked membrane glycoprotein that is also known as the LPS receptor. CD14 is expressed at high levels on monocytes and macrophages, and at lower levels on granulocytes. Some dendritic cell populations such as interfollicular dendritic cells, reticular dendritic cells, and Langerhans cells have also been reported to express CD14. As a high-affinity receptor for LPS, CD14 is involved in the clearance of gram-negative pathogens and in the upregulation of adhesion molecules and cytokine expression in monocytes and neutrophils.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human peripheral blood monocytes.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Fridlender ZG, et al. 1999. Hum. Immunol. 11:1028.
  2. Devitt A, et al. 1998. Nature 6675:505.
Product Citations
  1. Chabi S, et al. 2020. Cell Reports. 29(8):2307-2320.e6.. PubMed
  2. Lee PY, et al. 2020. Journal of Allergy and Clinical Immunology. 146(5):1194-1200.e1. PubMed
  3. Wu J, et al. 2022. Nat Commun. 13:676. PubMed
  4. Sonoda S, et al. 2022. STAR Protoc. 3:101386. PubMed
  5. Du Y, et al. 2022. FASEB J. 36:e22248. PubMed
  6. Xu Q, et al. 2022. Front Med (Lausanne). 9:940554. PubMed
  7. Karantanos T, et al. 2022. Haematologica. :. PubMed
  8. Bediaga NG, et al. 2022. Diabetes. 71:566. PubMed
  9. Lao L, et al. 2023. Cancer Immunol Res. 11:320. PubMed
  10. Piede N, et al. 2023. Cells. 12:. PubMed
  11. Laine A, et al. 2023. Microorganisms. 11:. PubMed
  12. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  13. Wang H, et al. 2022. Front Immunol. 13:852436. PubMed
  14. Ali S, et al. 2018. Purinergic Signal. 14:371. PubMed
  15. Gärtner K, et al. 2018. Cancer Med. 11:39. PubMed
  16. Terenzi DC, et al. 2021. STAR Protoc. 2:100311. PubMed
  17. Ugawa M, et al. 2021. Elife. 10:. PubMed
  18. Hu H, et al. 2022. Exp Ther Med. 24:487. PubMed
  19. Keenan CR, et al. 2020. Blood. 135:2049. PubMed
  20. Aagaard K, et al. 2017. Sci Rep. 7:41389. PubMed
  21. Bediaga NG, et al. 2021. Sci Rep. 0.825. PubMed
  22. Rodriguez A, et al. 2021. Cell Stem Cell. 28:33. PubMed
  23. Chen R, et al. 2021. Front Oncol. 11:743050. PubMed
RRID
AB_2565887 (BioLegend Cat. No. 367103)
AB_2565887 (BioLegend Cat. No. 367104)

Antigen Details

Structure
GPI-linked membrane glycoprotein.
Distribution

Monocytes, macrophages, dendritic cells, and granulocytes.

Function
LPS receptor, clearance of Gram-negative pathogens.
Interaction
LPS.
Ligand/Receptor
LPS.
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes, Neutrophils
Biology Area
Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References
  1. Stocks SC, et al. 1990. Biochem. J. 268:275.
  2. Wright SD, et al. 1990. Science 4975:1431.
Gene ID
929 View all products for this Gene ID
UniProt
View information about CD14 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 11/14/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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