Human peripheral blood mononuclear cells were treated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423302). Cells were surface stained with anti-human CD154 (clone W20199I) PE (left) or rat IgG2b, κ PE isotype control (right). Cells were costained with anti-human CD4 APC.
Human peripheral blood mononuclear cells were treated with Cell Activation Cocktail (without Brefeldin A) (Cat. No. 423302). Cells were surface stained with anti-human CD154 (clone W20199I) PE (left) or rat IgG2b, κ PE isotype control (right). Cells were costained with anti-human CD4 APC.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
Excitation Laser
Blue Laser (488 nm) Green Laser (532 nm)/Yellow-Green Laser (561 nm)
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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