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PE anti-human CD195 (CCR5) Phospho (Ser349) Antibody

Pricing & Availability
Clone
E11/19 (See other available formats)
Regulatory Status
RUO
Other Names
CD195, CCR5, C-C chemokine receptor type 5, HIV-1 fusion co-receptor
Isotype
Mouse IgG1, κ
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Product Citations
publications
E11_PE_042606
PMA (100ng/ml)-stimulated for 5 min (green histogram) and non-stimulated (red opened histogram) human peripheral blood lymphocytes intracellular stained with E11/19 PE
  • E11_PE_042606
    PMA (100ng/ml)-stimulated for 5 min (green histogram) and non-stimulated (red opened histogram) human peripheral blood lymphocytes intracellular stained with E11/19 PE
See PE spectral data
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321606 100 tests $317
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Description

CD195, also known as CCR5, is a 45 kD G protein-coupled seven transmembrane CC-chemokine receptor. It binds to MIP-1α, MIP-1β, and RANTES and is expressed on a subset of T cells and monocytes. CD195 mediates an intracellular signal thought to induce cell differentiation and proliferation. CCR5 has also been shown to act as a co-receptor for R5 HIV-1 cell entry; modification of CCR5 by sulfation contributes to the efficiency of HIV-1 entry.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Phosphopeptide (CEAPERA(pS)(pS)VYTR(pS)TGEQI(pS)VGL) of CCR5
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
Storage & Handling
The CD195 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The E11/19 monoclonal antibody binds to phosphoserine 349 corresponding to 22 c-terminal amino acid residues of human CCR5 and is useful for flow cytometric analysis, immunoblotting, and ELISA assay of CCR5 expression. Additional reported applications (for the relevant formats) include: immunofluorescence microscopy1, and ELISA detection1 when paired with T21/8 as the capture antibody (Cat. No. 321401/321402).

Application References

(PubMed link indicates BioLegend citation)
  1. Pollok-Kopp B, et al. 2003. J. Biol. Chem. 278:2190. (IF ELISA)
  2. Fox JM, et al. 2011. 117:1851. PubMed
  3. Scalley-Kim ML, et al. 2012. PLoS One. 7:e43332. PubMed.
Product Citations
  1. Dahl M, et al. 2008. J Pharmacol Exp Ther. 327:926. PubMed
  2. Huffman A,et al. 2017. Biol Blood Marrow Transplant. . 10.1016/j.bbmt.2017.10.028. PubMed
  3. Scalley-Kim M, et al. 2012. PLoS One. 7:e43332. PubMed
RRID
AB_528973 (BioLegend Cat. No. 321606)

Antigen Details

Structure
G-coupled receptor family 1, membrane protein, 45 kD
Distribution

Subset of T cells, monocytes

Function
Binds C-C chemokines and transduces an intracellular signal thought to result in proliferation and differentiation. Acts as a co-receptor with CD4 for HIV-1.
Ligand/Receptor
MIP-1a, MIP-1ß, RANTES
Cell Type
Monocytes, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR, Phospho-Proteins
Antigen References

1. Samson M, et al. 1996. Biochemistry 35:3362.
2. Raport CJ, et al. 1996. J. Biol. Chem. 271:17161.
3. Combadiere C, et al. 1996. J. Leukoc. Biol. 60:147.
4. Deng H, et al. 1996. Nature 381:661.

Gene ID
1234 View all products for this Gene ID
UniProt
View information about CD195 Phospho Ser349 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Does staining at room temperature or even at 37°C help for checking chemokine receptors expression?

Due to continuous recycling of many chemokine receptors, it may be worthwhile to consider staining at room temperature or at 37°C if the staining at lower temperature (which can potentially reduce receptor turnover) is not optimal.

Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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