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PE anti-human CD244 (2B4) Antibody

Pricing & Availability
Clone
C1.7 (See other available formats)
Regulatory Status
RUO
Workshop
VII 70350
Other Names
2B4, SLAMF4
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
C1dot7_PE_011409.jpg
Human peripheral blood lymphocytes stained with C1.7 PE
  • C1dot7_PE_011409.jpg
    Human peripheral blood lymphocytes stained with C1.7 PE
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329507 25 tests $122
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329508 100 tests $268
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Description

CD244, known as 2B4, is a 38 kD type I transmembrane protein. It is a member of the CD2 subset of the immunoglobulin superfamily (IgSF) molecules. CD244 is expressed on NK cells, a subset of T cells (including most CD8+ T cells and γ/δ T cells), monocytes, basophils, and eosinophils. CD48 is the ligand of CD244. It has been reported that ligation of human CD244 results in enhanced NK cell cytotoxicity and cytokine production. Recent studies have shown that human CD244, like murine CD244, has both activating and inhibitory functions, which are dependent on the density of surface 2B4 expression, degree of ligation, and the level of the adaptor molecule SAP expression.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: inducing cytokine production by NK cells, enhancing NK cytotoxicity, and Western blotting.

Application References

(PubMed link indicates BioLegend citation)
  1. Valiante NM and Trinchieri G. 1993. J. Exp. Med. 178:1397.
  2. Chuang SS, et al. 2000. Immunology. 100:378.
  3. Hoffmann SC, et al. 2011. J. Immunol. 186:2757. PubMed
  4. Rossi LE, et al. 2012. J Leukoc Biol. 91:321. PubMed
  5. Correia DV, et al. 2011. Blood 118:992. (FC) PubMed
Product Citations
  1. Zhong J, et al. 2018. Sci Adv. 4:eaas9864. PubMed
  2. Wei F, et al. 2013. Proc Natl Acad Sci U S A. 110:2480. PubMed
  3. Rossi L, et al. 2012. J Leukoc Biol. 91:321. PubMed
  4. Wu X, et al. 2021. Front Immunol. 12:731767. PubMed
  5. Haubner S, et al. 2019. Leukemia. 33:64. PubMed
RRID
AB_1279190 (BioLegend Cat. No. 329507)
AB_1279190 (BioLegend Cat. No. 329508)

Antigen Details

Structure
38 kD type I transmembrane protein, CD2 family member, Ig superfamily
Distribution

NK, T subset, monocytes, basophils, and eosinophils

Function
Activation and inhibition of NK cell function
Ligand/Receptor
CD48
Cell Type
Basophils, Eosinophils, Monocytes, NK cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules
Antigen References

1. Lee KM, et al. 2003. J. Immunol. 170:4881.
2. Chlewicki LK, et al. 2008. J. Immunol. 180:8159.
3. Messmer B, et al. 2006. J. Immunol. 176:4646.
4. Boles KS, et al. 2001. Immunol. Rev. 181:234.

Gene ID
51744 View all products for this Gene ID
UniProt
View information about CD244 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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