- Clone
- QA18A71 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- S152, T14, TNFRSF7
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![QA18A71_PE_CD27_FC_052324 QA18A71_PE_CD27_FC_052324](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/QA18A71_PE_CD27_FC_052324-.png&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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Human peripheral blood mononuclear cells were stained with anti-human CD3 (clone UCHT1) APC and anti-human CD27 recombinant (clone QA18A71) PE (left), or mouse IgG1, κ PE isotype control (right). Data shown are gated on the lymphocyte population.
Cat # | Size | Price | Quantity Check Availability | Save | ||
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321453 | 25 tests | $80 | ||||
321454 | 100 tests | $220 |
CD27, also known as S152 and T14, is a 50-55 kD type I membrane protein. It is a lymphocyte-specific member of the TNF-receptor superfamily. CD27 is expressed on medullary thymocytes, virtually all mature T cells, some B cells, and NK cells. CD27 binds to CD70, and plays a role in co-stimulation of T cell activation and regulation of B cell differentiation and proliferation. The cytoplasmic domains of CD27 have also been shown to interact with TRAF2 and TRAF5 to elicit NF-κB and SAPK/JNK activation.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
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FC - Quality tested
- Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
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Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
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Clone QA18A71 can completely block the binding of clone O323 on target cells.
Antigen Details
- Structure
- TNF-R superfamily, type I transmembrane glycoprotein, 50-55 kD
- Distribution
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Medullary thymocytes, T and B cell subsets, NK cells
- Function
- CD27 plays an important role in costimulation of T cell activation and regulation of B cell differentiation and proliferation.
- Interaction
- TRAF2, TRAF5
- Ligand/Receptor
- CD70
- Cell Type
- B cells, NK cells, Thymocytes
- Biology Area
- Costimulatory Molecules, Immunology
- Molecular Family
- CD Molecules
- Antigen References
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- Knapp W, et al. 1989. Leucocyte Typing IV: White Cell Differentiation Antigens. Oxford University Press.
- Schlossman S, et al. 1995. Leucocyte Typing V: White Cell Differentiation Antigens. Oxford University Press.
- Hintzen RQ, et al. 1994. Immunol Today. 15:307-11.
- Agematsu K, et al. 1995. J Immunol. 154:3627-35.
- Gene ID
- 939 View all products for this Gene ID
- UniProt
- View information about CD27 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All CD27 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
PE anti-human CD27 Recombinant Antibody | QA18A71 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-human CD27 Recombinant Antibody
Human peripheral blood mononuclear cells were stained with a...
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