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PE anti-human CD328 (Siglec-7) Antibody

Pricing & Availability
Clone
S7.7 (See other available formats)
Regulatory Status
RUO
Other Names
QA79 membrane protein, Adhesion inhibitory receptor molecule 1, p75/AIRM1, D-siglec
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
a-S7-7_PE_CD328_Antibody_1_102324
Human peripheral blood lymphocytes stained with anti-human CD8 PerCP/Cyanine5.5 and S7.7 PE
  • a-S7-7_PE_CD328_Antibody_1_102324
    Human peripheral blood lymphocytes stained with anti-human CD8 PerCP/Cyanine5.5 and S7.7 PE
  • b-S7dot7_PE_0305102
    Human peripheral blood monocytes stained with S7.7 PE
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347703 25 tests $177
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Description

Siglec-7 is a 75 kD type I transmembrane protein and a member of the family of sialic acid-binding immunoglobulin-like lectins (Siglecs), also known as p75/AIRM1. It is primarily found on NK cells and monocytes. The cytoplasmic domain of Siglec-7 contains immunoreceptor tyrosine-based inhibitory motif (ITIM). CD328 mediates sialic acid-dependent cell-cell binding and functions as an inhibitory receptor of NK cells. CD328 preferentially binds to sialylated glycans with α2,8 disialyl and α2,6 sialyl residues.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Siglec-7 3T3 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: Blocking2.

Application References

(PubMed link indicates BioLegend citation)
  1. Nicoll G, et al. 1999. J. Biol Chem. 274:34089.
  2. Hudak J, et al. 2014. Nat. Chem Biol. 10:69–75.
RRID
AB_2189410 (BioLegend Cat. No. 347703)

Antigen Details

Structure
Type I transmembrane protein, Siglec family member, 75 kD
Distribution

Monocytes, NK cells

Function
Inhibitory receptor of NK cells
Interaction
After tyrosine phosphorylation interacts with PTPN6 and SHP-1
Ligand/Receptor
Sialylated glycans (with α,8 disialyl and α2,6 sialyl residues)
Bioactivity
Inhibition of signal transduction by recruiting cytoplasmic phosphatases
Cell Type
Monocytes, NK cells
Biology Area
Immunology
Molecular Family
Adhesion Molecules, CD Molecules, Siglec Molecules
Antigen References

1. Avril T, et al. 2006. Infect Immun. 74:4133.
2. Avril T, et al. 2006. J. Leukoc. Biol. 80:787.
3. Orr SJ, et al. 2007. J. Biol Chem. 282:3418.
4. Brunetta E, et al. 2009. Blood 114:3822.

Gene ID
27036 View all products for this Gene ID
UniProt
View information about CD328 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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