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PE anti-human CD44 Antibody

Pricing & Availability
Clone
BJ18 (See other available formats)
Regulatory Status
RUO
Workshop
VI A034
Other Names
Hermes, Pgp-1, H-CAM, HUTCH-1, ECMR III, gp85, Ly-24
Isotype
Mouse IgG1, κ
Ave. Rating
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Product Citations
publications
BJ18_PE_030209.jpg
Human peripheral blood lymphocytes stained with BJ18 PE
  • BJ18_PE_030209.jpg
    Human peripheral blood lymphocytes stained with BJ18 PE
  • BJ18_PE_CD44_Antibody_042922
    Pre-lysed human blood leukocytes were stained with CD44 (clone BJ18) PE (filled histogram) or mouse IgG1, κ PE isotype control (open histogram).

    Data was acquired on the Moxi Flow, exported, and processed using FlowJo software.
Compare all formats See PE spectral data
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338807 25 tests $129
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338808 100 tests $304
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Description

CD44 is a 80-95 kD glycoprotein also known as Hermes, Pgp1, H-CAM, or HUTCH. It is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As B and T cells become activated or progress to the memory stage, CD44 expression increases from a low or mid level of intensity to high expression levels. Thus, CD44 has been reported to be a valuable marker for memory cell subsets. CD44 is an adhesion molecule involved in leukocyte attachment to and rolling on endothelial cells, homing to peripheral lymphoid organs and to the sites of inflammation, and leukocyte aggregation.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
African Green, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Normal human PBL
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Kishimoto T, et al. eds. 1997 Leucocyte Typing VI:White Cell Differentiation Antigen. Garland Publishing Inc.
Product Citations
  1. Qiao X, et al. 2021. Elife. 10: . PubMed
  2. Park J, et al. 2021. Exp Ther Med. 22:808. PubMed
  3. Labedz-Maslowska A, et al. 2021. Cells. 10: . PubMed
  4. Hasegawa K, et al. 2022. Cells. 11: . PubMed
  5. Robb KP, et al. 2022. Front Immunol. 13:972095. PubMed
  6. Gomez-Gallegos AA, et al. 2023. Stem Cell Res Ther. 14:16. PubMed
  7. Park J, et al. 2022. Bone Joint Res. 11:777. PubMed
  8. Kameishi S, et al. 2023. Sci Rep. 13:4421. PubMed
  9. Diessner J, et al. 2014. Cell Death Dis. 5:1149. PubMed
  10. Liu MY, et al. 2019. Oncol Lett. 18:3664. PubMed
  11. Palamides P, et al. 2016. Dis Model Mech. 9: 985 - 997. PubMed
  12. Zeng F, et al. 2021. Cancer Gene Therapy. . PubMed
  13. Murata K, et al. 2019. Oncol Lett. 17:5830. PubMed
  14. Floyd T, et al. 2011. J Immunol. 186:2033. PubMed
  15. Meng YM, et al. 2021. Nat Commun. 12:6011. PubMed
  16. Cromwell EF, et al. 2022. SLAS Discov. . PubMed
  17. Kolodziej M, et al. 2019. Adipocyte. 0.509722222. PubMed
  18. Ross NT, et al. 2020. Nature Chemical Biology. 16(1):50-59.. PubMed
  19. Jiang L, et al. 2020. Cell. 183(5):1219-1233.e18. PubMed
  20. Kolchakova D, et al. 2021. Biomolecules. 11:. PubMed
  21. Labedz-Maslowska A, et al. 2020. Int J Mol Sci. 21:. PubMed
  22. Liu Y, et al. 2018. Chin J Cancer. 37:2. PubMed
  23. Wei X, et al. 2019. Int J Mol Med. 44:1425. PubMed
  24. Wang G, et al. 2018. Int J Mol Med. 41:791. PubMed
RRID
AB_2260222 (BioLegend Cat. No. 338807)
AB_2260222 (BioLegend Cat. No. 338808)

Antigen Details

Structure
Variable splicing of CD44 gene generates many CD44 isoforms, 85 kD
Distribution

All leukocytes, epithelial cells, endothelial cells, hepatocytes, mesenchymal cells

Function
Leukocyte attachment and rolling on endothelial cells, stromal cells and ECM
Ligand/Receptor
Hyaluronan, MIP-1β, fibronectin, collagen
Cell Type
Endothelial cells, Epithelial cells, Leukocytes, Mesenchymal cells, Mesenchymal Stem Cells, Tregs
Biology Area
Cell Adhesion, Cell Biology, Immunology, Stem Cells
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. Haynes BF, et al. 1991. Cancer Cells 3:347.
3. Goldstein LA, et al. 1989. Cell 56:1063.
4. Mikecz K, et al. 1995. Nat. Med. 1:558.

Gene ID
960 View all products for this Gene ID
UniProt
View information about CD44 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 07/18/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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