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PE anti-human CXCL5 Antibody

Pricing & Availability
Clone
J111B7 (See other available formats)
Regulatory Status
RUO
Other Names
ENA-78, ENA78, epithelial-derived neutrophil-activating protein 78, small inducible cytokine B5
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
J111B7_PE_CXCL5_Antibody_FC_1_080213.jpg
Human peripheral blood monocytes were stimulated with LPS (overnight, in the presence of monensin), fixed and permeabilized and then stained with CD14 APC (clone HCD14) and CXCL5 (clone J111B7) PE (top) or mouse IgG2b, κ PE isotype control (bottom).
  • J111B7_PE_CXCL5_Antibody_FC_1_080213.jpg
    Human peripheral blood monocytes were stimulated with LPS (overnight, in the presence of monensin), fixed and permeabilized and then stained with CD14 APC (clone HCD14) and CXCL5 (clone J111B7) PE (top) or mouse IgG2b, κ PE isotype control (bottom).
  • J111B7_PE_CXCL5_Antibody_FC_2_080213.jpg
See PE spectral data
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524103 25 tests $153
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524104 100 tests $360
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Description

CXCL5, also known as ENA-78, is a 11.9 kD chemokine and belongs to the intercrine alpha, CXC chemokine family. It is expressed by monocytes and eosinophils in response to IL-1 and TNFα. CXCL5 can interact with CXCR2 and activate neutrophils and induce their migration. In vitro neutrophil chemotactic activity of CXCL5 is three fold higher. IFNγ suppresses expression of CXCL5. NFκB alone is sufficient to induce CXCL5 in contrast to IL-8, which requires both NFκB and C/EBP. Two forms are produced by N terminal proteolytic cleavage, ENA78 (8-78) and ENA 78 (9-78), and these isoforms exhibit higher chemotactic activity. Increased intra-hepatic levels of CXCL5 have been reported in patients with chronic liver disease.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant Human CXCL5 (ENA-78)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2562377 (BioLegend Cat. No. 524103)
AB_2562377 (BioLegend Cat. No. 524104)

Antigen Details

Structure
CXC chemokine family, 11.9 kD
Distribution
Monocytes, platelets, and eosinophils
Function
Interacts with CXCR2 and is involved in neutrophil activation and chemotaxis for HIV-1
Ligand/Receptor
CXCR2
Cell Type
Monocytes, Platelets, Eosinophils
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Walz A, et al. 1991. J. Exp. Med. 174:1355.
2. Power CA, et al. 1994. Gene. 151:333.
3. Corbett MS, et al. 1994. Biochem. Biophys. Res. Commun. 205:612.

Gene ID
6374 View all products for this Gene ID
UniProt
View information about CXCL5 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 08/15/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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