- Clone
- 3A3-N2 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Interleukin 21
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
![A_3A3-N2_PE_091509 A_3A3-N2_PE_091509](https://d1spbj2x7qk4bg.cloudfront.net/Admin/Public/GetImage.ashx?Image=/Files/Images/media_assets/products/product_images/A_3A3-N2_PE_091509.jpg&Width=240&Height=300&altFmImage_path=&Compression=90&Crop=5)
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PMA+ionomycin-stimulated (5 hours) human PBMCs intracellular stained with 3A3-N2 PE and CD3 APC (top panel), bottom data shows the staining of 3A3-N2 PE can be blocked by preincubation of the cells with purified 3A3-N2 -
Cat # | Size | Price | Quantity Check Availability | Save | ||
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513003 | 25 tests | $171 | ||||
513004 | 100 tests | $341 |
Interleukin 21 (IL-21) is a potent immunomodulatory cytokine mainly produced by NKT and CD4+ T-cells, particularly the inflammatory Th17 subset, and has pleiotropic effects on both innate and adaptive immune responses. These actions include positive effects such as enhancing proliferation of NK cells and cytotoxic T cells, and inhibitory effects on the antigen-presenting function of dendritic cells. It can also be proapoptotic for B cells and NK cells. Studies have shown that IL-21 is also an autocrine cytokine that potently induces Th17 differentiation, suppresses Foxp3 expression, and serves as a target for treating inflammatory diseases.
Product DetailsProduct Details
- Verified Reactivity
- Human
- Reported Reactivity
- Rhesus
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant full length human IL-21
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
ICFC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Product Citations
-
- RRID
-
AB_1877230 (BioLegend Cat. No. 513003)
AB_2249025 (BioLegend Cat. No. 513004)
Antigen Details
- Distribution
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Th17
- Function
- Immunomodulation
- Cell Sources
- NK-T cells and T cells
- Cell Targets
- Cytotoxic T cells (CTL) and Natural Killer (NK) cells that can destroy malignant or infected cells
- Receptors
- IL-21R
- Cell Type
- Th17
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- Cytokines/Chemokines
- Antigen References
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1. Nurieva R. 2007. Nature 448:416.
2. Parrish-Novak J, et al. 2002. J. Leukocyte Biol. 72:856.
3. Dumoutier L, et al. 2000. Proc. Natl. Acad. Sci. USA 97:10144.
4. Asao H, et al. 2001. J. Immunol. 167:1.
5. Parrish-Novak J, et al. 2000. Nature 408:57. - Gene ID
- 59067 View all products for this Gene ID
- UniProt
- View information about IL-21 on UniProt.org
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All IL-21 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-human IL-21 | 3A3-N2 | ELISA Capture |
PE anti-human IL-21 | 3A3-N2 | ICFC |
Alexa Fluor® 647 anti-human IL-21 | 3A3-N2 | ICFC |
APC anti-human IL-21 | 3A3-N2 | ICFC |
Purified anti-human IL-21 (Maxpar® Ready) | 3A3-N2 | ELISA Capture,CyTOF® |
PerCP/Cyanine5.5 anti-human IL-21 | 3A3-N2 | ICFC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human IL-21
-
PE anti-human IL-21
PMA+ionomycin-stimulated (5 hours) human PBMCs intracellular... -
Alexa Fluor® 647 anti-human IL-21
PMA/ionomycin-stimulated (6 hours) human peripheral blood ly... -
APC anti-human IL-21
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
Purified anti-human IL-21 (Maxpar® Ready)
Human Th17-polarized CD4+ T cells were incubated for 5 hours... -
PerCP/Cyanine5.5 anti-human IL-21
PMA/ionomycin-stimulated (4 hours) human peripheral blood ly...
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