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PE anti-human IL-27/IL-35 EBI3 (monomer, dimer, heterodimer) Antibody

Pricing & Availability
Clone
B032F6 (See other available formats)
Regulatory Status
RUO
Other Names
Epstein Barr virus induced gene 3, IL-27B, IL-35 subunit
Isotype
Mouse IgG2b, κ
Ave. Rating
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Product Citations
publications
B032F6_PE_EBI3_Antibody_FC_1_111313
Human peripheral blood monocytes were stimulated with IFNγ, LPS, and R848 for 24 hours (in the presence of Brefeldin A). Cells were then fixed, permeabilized, and stained with CD14 APC and EBI3 (clone B032F6) PE (top) or mouse IgG2b, κ PE isotype control (bottom).
  • B032F6_PE_EBI3_Antibody_FC_1_111313
    Human peripheral blood monocytes were stimulated with IFNγ, LPS, and R848 for 24 hours (in the presence of Brefeldin A). Cells were then fixed, permeabilized, and stained with CD14 APC and EBI3 (clone B032F6) PE (top) or mouse IgG2b, κ PE isotype control (bottom).
  • B032F6_PE_EBI3_Antibody_FC_2_111313
See PE spectral data
Cat # Size Price Quantity Check Availability Save
360903 25 tests $146
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360904 100 tests $360
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Description

Epstein-Barr virus induced gene-3, also known as EBI3, is an IL-12 p40-related protein that can heterodimerize with IL-12 p35 to form IL-35, or with an IL-12 p35-related protein, p28, to form IL-27. It is produced by antigen-presenting cells such as B cells, monocytes, and dendritic cells following stimulation through TLR. It can regulate T cell differentiation and proliferation. Both IL-27 and IL-35 are anti-inflammatory cytokines that are able to inhibit Th17 generation, promote Th1 diferentiation, increase cytolytic potential of CD8+ T cells, and impact B cell differentiation and Ig production.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified human IL-27
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Alenazy MF, et al. 2021. Sci Rep. 11:5629. PubMed
  2. Khanam A, et al. 2021. Front Immunol. 11:599648. PubMed
  3. Wang K, et al. 2018. Medicine (Baltimore). 97:e0710. PubMed
RRID
AB_2562880 (BioLegend Cat. No. 360903)
AB_2562880 (BioLegend Cat. No. 360904)

Antigen Details

Structure
Hematopoietin receptor family, subunit of IL-27 and IL-35
Distribution

B cells, monocytes and dendritic cells

Function
Pro- and anti-inflammatory
Interaction
IL-27 p28 subunit, IL-35 p35 subunit
Cell Type
B cells, Dendritic cells, Monocytes
Biology Area
Immunology, Innate Immunity
Molecular Family
Cytokines/Chemokines
Antigen References

1. Pflanz S, et al. 2002. Immunity. 16: 779.
2. Takeda A, et al. 2003. J. immunol. 170: 4886.
3. Pflanz S, et al. 2004. J. Immunol. 172: 2225.
4. Li MF, et al. 2013. Dev. Comp. Immunol. 41: 514.

Gene ID
10148 View all products for this Gene ID
UniProt
View information about IL-27 IL-35 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 12/05/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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