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PE anti-human SSEA-4 Antibody

Pricing & Availability
Clone
MC-813-70 (See other available formats)
Regulatory Status
RUO
Other Names
Stage-Specific Embryonic Antigen-4, SSEA4
Isotype
Mouse IgG3, κ
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Product Citations
publications
MC-813-70_PE_061808.jpg
Human teratocarcinoma cell line, NCCIT, stained with MC-813-70 PE
  • MC-813-70_PE_061808.jpg
    Human teratocarcinoma cell line, NCCIT, stained with MC-813-70 PE
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330405 25 tests $153
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330406 100 tests $275
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Description

The MC813-70 antibody reacts with a carbohydrate antigen which is named as Stage-Specific Embryonic Antigen-4 (SSEA-4). It is a terminal structure of globo-series glycolipids (NeuAcα2-3Galβ1-3GalNAcβ1-R). SSEA-4 is expressed on human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), and preimplantation embryos, subset of mesenchymal stem cells, erythrocytes, and erythromyeloid leukemia cell line K562. The expression of SSEA-3 on human ESC is downregulated upon differentiation. SSEA-4 is also expressed by mouse on unfertilized eggs and preimplantation to early cleaved embryos.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human
Reported Reactivity
Mouse, Baboon, Cynomolgus, Rhesus
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human embryonic carcinoma cell line 2102Ep
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA); may contain stabilizer.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: ELISA1 and immunohistochemistry1 of acetone-fixed frozen tissue sections and formalin-fixed paraffin-embedded sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Kannagi R, et al. 1983. The EMBO J. 2:2355
Product Citations
  1. Shimoda Y, et al. 2022. Stem Cell Res. 62:102793. PubMed
  2. Tamaoki N, et al. 2023. Cell Rep Methods. 3:100460. PubMed
  3. Nomi K, et al. 2021. Cell Reports. 34(5):108715. PubMed
  4. Hua Y, et al. 2022. Stem Cell Res Ther. 13:223. PubMed
  5. Chung K, et al. 2014. PLoS One. 9:95304. PubMed
  6. Niwa A, et al. 2011. PLoS One. 6:e22261. PubMed
  7. Dor L, et al. 2021. Stem Cell Research. 55:102495. PubMed
  8. Kimura Y, et al. 2020. Stem Cell Research. 72.69236111. PubMed
  9. Zhao M, et al. 2021. Ann Transl Med. 9:1476. PubMed
  10. Wang Y, et al. 2021. Biomaterials. 265:120387. PubMed
  11. Garcia-Montero A, et al. 2016. Blood. 127: 761 - 768. PubMed
  12. Beylina A, et al. 2021. Stem Cell Research. 53:102354. PubMed
  13. Shibata S et al. 2018. Cell reports. 25(6):1668-1679 . PubMed
  14. Klaihmon P, et al. 2021. Stem Cell Research. 55:102472. PubMed
  15. Bi Y, et al. 2020. Cell Reports. 30(11):3917-3931. PubMed
  16. Tanaka Y et al. 2018. Stem cell research. 28:100-104 . PubMed
  17. Tanaka Y et al. 2018. Stem Cell Res. 31:11-15 . PubMed
  18. Zheng Y, et al. 2014. Hum Reprod. 29:2497. PubMed
  19. Pan C, et al. 2019. Int J Mol Med. 44:1629. PubMed
  20. Wei X, et al. 2019. Int J Mol Med. 44:1425. PubMed
  21. Wang G, et al. 2018. Int J Mol Med. 41:791. PubMed
RRID
AB_1089206 (BioLegend Cat. No. 330405)
AB_1089206 (BioLegend Cat. No. 330406)

Antigen Details

Structure
Carbohydrate epitope on glycosphingolipids
Distribution

Human teratocarcinoma cells (EC), embryonic stem cells (ESC), embryonic germ cells (EG), preimplantation embryos, and erythrocytes; mouse oocytes, early cleaved embryos

Function
Regulation of differentiation
Cell Type
Embryonic Stem Cells, Mesenchymal Stem Cells, Neural Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Antigen References

1. Kannagi R, et al. 1983. The EMBO J. 2:2355
2. Henderson JK, et al. 2002. Stem Cells. 20:329
3. Brimble SN, et al. 2007. Stem Cells. 25:54
4. Battula VL, et al. 2007. Differentiation. 75:279

Gene ID
NA
UniProt
View information about SSEA-4 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 03/26/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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