Analyte Specific Reagent. Analytical and performance characteristics are not established.
- Clone
- MAb11
- Other Names
- Tumor necrosis factor-α, Cachectin, Necrosin, Macrophage cytotoxic factor (MCF), Differentiation inducing factor (DIF), TNFSF2
- Isotype
- Mouse IgG1, κ
Cat # | Size | Price | Quantity Check Availability | Save | ||
---|---|---|---|---|---|---|
986802 | 500 µL | $322 |
TNF-α is secreted by macrophages, monocytes, neutrophils, T cells, and NK cells. Many transformed cell lines also secrete TNF-α. Monomeric human TNF-α is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kD. TNF-α forms multimeric complexes; stable trimers are most common in solution. A 26 kD membrane form of TNF-α has also been described. TNF-α binding to surface receptors elicits a wide array of biological activities including: cytolysis and cytostasis of many tumor cell lines in vitro, hemorraghic necrosis of tumors in vivo, increased fibroblast proliferation, and enhanced chemotaxis and phagocytosis in neutrophils.
Product DetailsProduct Details
- Reactivity
- Human
- Formulation
- Phosphate-buffered solution, pH7.2, 0.09% sodium azide, 0.2% (w/v) BSA (origin USA), and a stabilizer.
- Preparation
- The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
- Concentration
- 100 µg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
Suggested for Intracellular Staining for Flow Cytometry
- Disclaimer
-
WARNINGS AND PRECAUTIONS
- Use appropriate personal protective equipment and safety practices per universal precautions when working with this reagent. Refer to the reagent safety data sheet.
- This antibody contains sodium azide. Follow federal, state and local regulations to dispose of this reagent. Sodium azide build-up in metal wastepipes may lead to explosive conditions; if disposing of reagent down wastepipes, flush with water after disposal.
- All specimens, samples and any material coming in contact with them should be considered potentially infectious and should be disposed of with proper precautions and in accordance with federal, state and local regulations.
- Do not use this reagent beyond the expiration date stated on the label.
- Do not use this reagent if it appears cloudy or if there is any change in the appearance of the reagent as these may be an indication of possible deterioration.
- Avoid prolonged exposure of the reagent or stained cells to light.
Antigen Details
- Antigen References
-
- Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press, San Diego.
- Beutler B, et al. 1988. Annu Rev Biochem. 57:505-18.
- Beutler B, et al. 1989. Annu Rev Immunol. 7:625-55.
- Tracey K, et al. 1993. Crit Care Med. 21:S415-22.
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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APC anti-human TNF-α
PMA/ionomycin-stimulated (6 hours) human peripheral blood ly... PMA/ionomycin-stimulated (6 hours) human peripheral blood ly... -
Biotin anti-human TNF-α
Human PBMCs, stimulated with 1 µg/mL of LPS for 8 h and trea... -
FITC anti-human TNF-α
PMA/ionomycin-stimulated (6 hours) human peripheral blood ly... -
PE anti-human TNF-α
PMA/Ionomycin-stimulated human PBMCs were stained with CD3 P... -
Purified anti-human TNF-α
-
Alexa Fluor® 488 anti-human TNF-α
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
Alexa Fluor® 647 anti-human TNF-α
PMA/ionomycin-stimulated (6 hours) human peripheral blood ly... PMA/ionomycin-stimulated (6 hours) human peripheral blood ly... -
Alexa Fluor® 700 anti-human TNF-α
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
Pacific Blue™ anti-human TNF-α
PMA/ionomycin-stimulated (6 hours) human peripheral blood ly... PMA/ionomycin-stimulated (6 hours) human peripheral blood ly... -
PerCP/Cyanine5.5 anti-human TNF-α
PMA+ionomycin stimulated (6 hours) human peripheral blood ly... -
PE/Cyanine7 anti-human TNF-α
PMA+ionomycin-stimulated (6 hours) peripheral blood lymphocy... -
Brilliant Violet 421™ anti-human TNF-α
PMA+ionomycin stimulated (6 hours) human peripheral blood ly... -
Brilliant Violet 605™ anti-human TNF-α
PMA+ionomycin stimulated (6 hours) human peripheral blood ly... -
Brilliant Violet 650™ anti-human TNF-α
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
Brilliant Violet 711™ anti-human TNF-α
PMA+ ionomycin-stimulated (6 hours) human peripheral blood l... -
APC/Cyanine7 anti-human TNF-α
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
Purified anti-human TNF-α (Maxpar® Ready)
Human PBMCs were incubated for 6 hours in media alone (botto... -
PE/Dazzle™ 594 anti-human TNF-α
PMA+ionomycin stimulated (6 hours) human peripheral blood ly... -
Brilliant Violet 785™ anti-human TNF-α
PMA + ionomycin stimulated (six hours) human peripheral bloo... -
Brilliant Violet 510™ anti-human TNF-α
PMA + ionomycin stimulated (six hours) human peripheral bloo... -
PerCP anti-human TNF-α
PMA + ionomycin stimulated (six hours) human peripheral bloo... -
PE anti-human TNF-α
PMA + Ionomycin with Brefeldin A stimulated (4-hour) human ... -
Spark Blue™ 515 anti-human TNF-α
PMA+ ionomycin stimulated (5 hours) human peripheral blood l... -
Spark UV™ 387 anti-human TNF-α
PMA+ionomycin-stimulated (6 hours) human peripheral blood ly... -
TotalSeq™-B0945 anti-human TNF-α
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Brilliant Violet 750™ anti-human TNF-α
PMA + lonomycin stimulated (6 hours) human peripheral blood ... -
APC/Fire™ 810 anti-human TNF-α
Human peripheral blood lymphocytes were stimulated with (lef... -
GMP PE anti-human TNF-α
PMA + Ionomycin with Brefeldin A stimulated (4-hour) human p...
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