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PE anti-mouse CD120b (TNF R Type II/p75) Antibody

Pricing & Availability
Clone
TR75-89 (See other available formats)
Regulatory Status
RUO
Other Names
TNFRII, TNFR type II, p75, TNFR80, TNFRSF1B
Isotype
Armenian Hamster IgG
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Product Citations
publications
TR75-89_PE_083107
C57BL/6 mouse splenocytes stained with TR75-89 PE
  • TR75-89_PE_083107
    C57BL/6 mouse splenocytes stained with TR75-89 PE
Compare all formats See PE spectral data
Cat # Size Price Quantity Check Availability Save
113405 50 µg $122
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113406 200 µg $345
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Description

CD120b is a 75 kD type I transmembrane protein, also known as Tumor Necrosis Factor Receptor Type II (TNFRII) or p75. It is expressed on a variety of cells at low levels; the expression is upregulated upon activation. This receptor binds both TNF-α and LT-α (also known as TNF-β). In association with TRAF1 and TRAF2, the receptor crosslinking induced by TNF-α or LT-α trimers is critical for signal transduction, leading to apoptosis, NF-kB activation, increased expression of proinflammatory genes, tumor necrosis, and cell differentiation depending on cell type and differentiation state.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
E. coli -expressed mouse Type II TNFR
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported applications (for the relevant formats) include: immunoprecipitation1. The TR75-89 antibody does not block TNF-α binding to receptor. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 113406) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 113404) or biotinylated anti-Armenian hamster IgG second step (Cat. No. 405501), followed by SAv-PE (Cat. No. 405204)).

Application References

(PubMed link indicates BioLegend citation)
  1. Sheehan KC, et al. 1995. J. Exp. Med. 181:607. (IP)
  2. Schlaepfer DD, et al. 2007. J. Biol. Chem. 282:17450. (FC)
  3. Bell JH, et al. 2007. J. Leukoc. Biol. 82:173. (FC)
Product Citations
  1. Rohwedder I, et al. 2023. JCI Insight. 8: . PubMed
  2. Ohta S, et al. 2011. PLoS One. 6:e16182. PubMed
  3. Paris J et al. 2019. Cell Stem Cell. 25(1):137-148 . PubMed
  4. Baram T, et al. 2021. Cells. 10:. PubMed
  5. Lindborg JA et al. 2017. The Journal of Neuroscience. 37(43):10258-10277 . PubMed
  6. Akagi T, et al. 2022. Front Immunol. 13:926175. PubMed
  7. Sasaki K, et al. 2019. Nat Commun. 10:3878. PubMed
  8. Jiang Y, et al. 2019. J Immunother Cancer. 7:28. PubMed
  9. Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
  10. Mansouri S, et al. 2020. Mucosal Immunol. 0.954861111. PubMed
  11. Yamashita M, et al. 2019. Cell Stem Cell. 25:357. PubMed
  12. Akhtar MN, et al. 2020. PLoS Pathog. 16:e1009136. PubMed
RRID
AB_2206941 (BioLegend Cat. No. 113405)
AB_2206941 (BioLegend Cat. No. 113406)

Antigen Details

Structure
TNFR superfamily, 75 kD
Distribution

Variety of cell types at low levels

Function
Apoptosis, NF-κB activation, inflammation, tumor necrosis, cell differentiation
Ligand/Receptor
TNF-α, LT-α (TNF-β)
Cell Type
Tregs
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Aggarwal BB, et al. 1985. Nature 318 665.
2. Chan FKM, et al. 2000. Science 288:2351.
3. Loetscher H, et al. 1990. Cell 61:351.
4. Rothe J, et al. 1993. Nature 364:798.

Gene ID
21938 View all products for this Gene ID
UniProt
View information about CD120b on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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