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PE anti-mouse CD140a Antibody

Pricing & Availability
Clone
APA5 (See other available formats)
Regulatory Status
RUO
Other Names
PDGF receptor-α, PDGFR-α
Isotype
Rat IgG2a, κ
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Product Citations
publications
APA5_PE_CD140a_Antibody_071217
Mouse fibroblast NIH/3T3 cells were stained with CD140a (clone APA5) PE (filled histogram) or Rat IgG2a, κ PE isotype control (open histogram).
  • APA5_PE_CD140a_Antibody_071217
    Mouse fibroblast NIH/3T3 cells were stained with CD140a (clone APA5) PE (filled histogram) or Rat IgG2a, κ PE isotype control (open histogram).
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135905 50 µg $173
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135906 200 µg $426
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Description

Platelet-derived growth factor receptor-α (PDGFR-α), CD140a, is one of two receptors for platelet-derived growth factors (PDGFs) and binds to all isoforms of PDGFs: PDGF-AA, PDGF-AB, and PDGF-BB. PDGFRa is a receptor tyrosine kinase that forms homodimers or heterodimers on the surface upon ligand binding and phosphorylates substrates. PDGFRs consist of either homodimers of α/α, β/β, or heterodimers of α/β. PDGF receptors, α and β, are single glycoproteins with intracellular tyrosine kinase domain. Their ligand, PDGF, is a mitogen for connective tissue and glial cells. CD140a is expressed on embryonic tissues and mesenchymal-derived cells of adult mice. PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes, and neutrophils.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse PDGFR-α-hIgG1 recombinant fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported (for relevant formats) applications include: Western Blot, blocking function2, and immunohistochemical staining of paraffin and frozen sections. The LEAF™ purified antibody is recommended for functional assays.

Application References

(PubMed link indicates BioLegend citation)
  1. Takakura N, et al. 1996. J. Invest. Dermatol. 107:770.
  2. Liao C, et al. 2010. J. Clin. Invest. 120:242. (Block)
  3. Chen H, et al. 2015. ASN Neuro 8:7. PubMed.
  4. Miyawaki T, et al. 2004. J Neurosci. 24(37): 8124-34. (IHC-F)
Product Citations
  1. Stoupa A, et al. 2018. EMBO Mol Med. 10:. PubMed
  2. Gabitova-Cornell L, et al. 2020. Cancer Cell. 38(4):567-583.e11. PubMed
  3. Vercauteren Drubbel A, et al. 2021. Cell Stem Cell. . PubMed
  4. Zelic M, et al. 2021. Cell Reports. 35(6):109112. PubMed
  5. Buechler MB, et al. 2021. Nature. 593:575. PubMed
  6. Frondelli MJ, et al. 2022. J Neurosci Res. 100:578. PubMed
  7. Malolina EA, et al. 2022. Mol Reprod Dev. 89:243. PubMed
  8. Poh AR, et al. 2022. Cell Rep. 41:111479. PubMed
  9. Contreras O, et al. 2023. Front Cell Dev Biol. 11:1017660. PubMed
  10. He T, et al. 2023. iScience. 26:106289. PubMed
  11. Wagner G, et al. 2017. Sci Rep. 7:40881. PubMed
  12. Huang Z et al. 2017. Cell metabolism. 26(3):493-508 . PubMed
  13. Salzer MC et al. 2018. Cell. 175(6):1575-1590 . PubMed
  14. Chen M, et al. 2020. Sci Adv. 6:eaax9605. PubMed
  15. Wu R, et al. 2019. J Cell Mol Med. 24:1684. PubMed
  16. Biffi G, et al. 2018. Cancer Discov. 2:282. PubMed
  17. Cardot-Ruffino V, et al. 2020. Genesis. 58:e23359. PubMed
  18. Lu A, et al. 2020. Sci Rep. 10:7075. PubMed
  19. Joshi PA, et al. 2019. Nat Commun. 10:1760. PubMed
  20. Jakob L, et al. 2021. Int J Mol Sci. 22:. PubMed
  21. Frondelli MJ, et al. 2021. Neurotrauma Rep. 2:285. PubMed
  22. Jackson-Jones LH, et al. 2020. Immunity. 52:700. PubMed
  23. Diekmann U, et al. 2019. Sci Rep. 9:996. PubMed
  24. Cardot-Ruffino V, et al. 2020. Sci Rep. 10:3880. PubMed
  25. Ito K, et al. 2014. J Biol Chem. 289:29892. PubMed
  26. Derecka M, et al. 2020. Nat Immunol. 261:21. PubMed
  27. Runyan CE, et al. 2020. Aging Cell. 19:00. PubMed
  28. Frech S, et al. 2021. J Invest Dermatol. . PubMed
  29. Xi W, et al. 2021. Stem Cell Res Ther. 167:12. PubMed
  30. Oguri Y, et al. 2020. Cell. 182(3):563-577.e20. PubMed
  31. Nawaz A, et al. 2018. Sci Rep. 6:37. PubMed
  32. Matthew B Buechler et al. 2019. Immunity. 51(1):119-130 . PubMed
  33. Hasegawa T, et al. 2020. Sci Rep. 10:13480. PubMed
  34. Fung CW, et al. 2022. Cell Discov. 8:61. PubMed
  35. Kim DK, et al. 2022. Nat Commun. 13:6292. PubMed
  36. Haider N, et al. 2020. Cell Commun Signal. 18:26. PubMed
  37. Shibamoto M, et al. 2019. Int Heart J. 60:944. PubMed
  38. Buffolo M, et al. 2019. Cell Rep. 29:270. PubMed
  39. Steele NG, et al. 2021. Clin Cancer Res. 27:2023. PubMed
  40. Singhal P, et al. 2016. Proc Natl Acad Sci U S A. 113: 122 - 127. PubMed
  41. Konishi S, et al. 2022. STAR Protoc. 3:101447. PubMed
  42. Rai S, et al. 2022. Nat Commun. 13:5346. PubMed
  43. Maller O, et al. 2020. Nat Mater. 20:548. PubMed
  44. Liu J, et al. 2020. Cell Mol Gastroenterol Hepatol. . PubMed
  45. Hara T, et al. 2021. Cancer Cell. 39(6):779-792.e11. PubMed
  46. Chen Y, et al. 2021. Cancer Cell. 39(4):548-565.e6. PubMed
  47. Kwon H, Kim S 2016. Cell Death Dis. 7:22285. PubMed
  48. Burl RB et al. 2018. Cell metabolism. 28(2):300-309 . PubMed
  49. Mahmoudi S, et al. 2019. Nature. 574:553. PubMed
  50. Wang Y, et al. 2020. Cell Reports. 31(5):107600. PubMed
  51. Velloso FJ, et al. 2022. STAR Protoc. 3:101065. PubMed
  52. Velletri T, et al. 2020. Cell Death Differ. . PubMed
  53. Bravo JI, et al. 2021. STAR Protocols. 2(2):100406. PubMed
  54. Thomson CA, et al. 2018. J Immunol. 201:215. PubMed
  55. Ferrari N, et al. 2019. Nat Commun. 10:130. PubMed
RRID
AB_1953268 (BioLegend Cat. No. 135905)
AB_1953268 (BioLegend Cat. No. 135906)

Antigen Details

Structure
Alpha chain of the platelet-derived growth factor receptor, a receptor tyrosine kinase that forms homo- or hetero-dimers on the surface after ligand binding.
Distribution

Expressed on embryonic tissues and mesenchymal-derived cells of adult mouse.

Function
Play a role in wound healing and act as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils.
Ligand/Receptor
PDGFs
Cell Type
Embryonic Stem Cells, Mesenchymal cells, Mesenchymal Stem Cells
Biology Area
Cell Biology, Immunology, Neuroscience, Neuroscience Cell Markers, Stem Cells
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Mukouyama YS, et al. 2006. Proc Natl Acad Sci USA. 103(5):1551
2. Miyawaki T, et al. 2004. J Neurosci. 24(37):8124
3. Takakura N, et al. 1997. J Histochem Cytochem. 45(6):883

Gene ID
18595 View all products for this Gene ID
UniProt
View information about CD140a on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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