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PE anti-mouse CD154 (CD40L) Antibody

Pricing & Availability
Clone
SA047C3 (See other available formats)
Regulatory Status
RUO
Other Names
CD40L, gp39, TRAP, T-BAM, TNFSF5, CD40 Ligand
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
SA047C3_PE_CD154_Antibody_091019
Enriched BALB/c mouse splenic T cells were stimulated with PMA plus ionomycin for 6 hours and then stained with mouse CD154 (CD40L) (clone SA047C3) PE (flled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • SA047C3_PE_CD154_Antibody_091019
    Enriched BALB/c mouse splenic T cells were stimulated with PMA plus ionomycin for 6 hours and then stained with mouse CD154 (CD40L) (clone SA047C3) PE (flled histogram) or rat IgG2a, κ PE isotype control (open histogram).
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157003 25 µg $80
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157004 100 µg $250
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Description

CD154 has been kown a 39 kD TNF superfamily member also known as CD40 ligand, gp39, T-BAM, TRAP, and Ly-62. CD154 is an accessory molecule expressed predominantly on activated CD4+ lymphocytes that bind CD40. CD154 plays an important role in T-B cell costimulation and Ig class switching.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
mCD40L-transfectants
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Wang R, et al. 2022. J Immunother Cancer. 10:. PubMed
RRID
AB_2814083 (BioLegend Cat. No. 157003)
AB_2814083 (BioLegend Cat. No. 157004)

Antigen Details

Structure
A 39 kD TNF superfamily memeber
Distribution

Surface activated CD4+ T cells,B cells, macrophages, dendritic cells, mast cells, NK cells, polymorphs, monocytes, and activated platelets.

Function
Survival signaling to CD40+ B cells and Ig class switching.
Ligand/Receptor
CD40
Cell Type
B cells, Basophils, Dendritic cells, Fibroblasts, Macrophages, Mast cells, NK cells, Platelets, T cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Gene ID
959 View all products for this Gene ID
UniProt
View information about CD154 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 09/10/2019

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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