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PE anti-mouse CD163 Antibody

Pricing & Availability
Clone
S15049F (See other available formats)
Regulatory Status
RUO
Other Names
M130, MM130, SCARI1
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
S15049F_PE_CD163_Antibody_041620
C57BL/6 bone marrow cells were stained with CD11b APC and CD163 (clone S15049F) (left) PE, or rat IgG2a, κ PE isotype control (right). Data shown was gated on total bone marrow cells.
  • S15049F_PE_CD163_Antibody_041620
    C57BL/6 bone marrow cells were stained with CD11b APC and CD163 (clone S15049F) (left) PE, or rat IgG2a, κ PE isotype control (right). Data shown was gated on total bone marrow cells.
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156703 25 µg $141
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156704 100 µg $365
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Description

CD163 is a member of the group B scavenger receptor cysteine-rich superfamily, also known as GHI/61, M130, RM3/1, p155, hemoglobin-haptoglobin complex receptor, or macrophage-associated antigen. It is a 134 kD (non-reduced)/155 kD (reduced) glycoprotein primarily expressed on macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells. CD163 binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages. Membrane CD163 can be cleaved by metalloproteinases (MMP), resulting in a soluble form. Elevated serum level of sCD163 has been implicated in many kinds of inflammatory diseases.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant mouse CD163 extracellular domain
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Gu J, et al. 2023. J Cell Biochem. 124:557. PubMed
  2. Choconta JL, et al. 2023. Immun Ageing. 20:22. PubMed
  3. Xu M, et al. 2021. Front Physiol. 12:700338. PubMed
  4. Lee SS, et al. 2022. Commun Biol. 5:654. PubMed
RRID
AB_2860724 (BioLegend Cat. No. 156703)
AB_2860724 (BioLegend Cat. No. 156704)

Antigen Details

Structure
Type 1 transmembrane glycoprotein, 134 kD (non-reduced)/155 kD (reduced) glycoprotein
Distribution

Macrophages, Kupffer cells, monocytes, a subset of dendritic cells, and a subset of hematopoietic stem/progenitor cells

Function
Binds to haptoglobin-hemoglobin complex and TWEAK, and plays a role in clearing hemoglobin and regulating cytokine production by macrophages.
Cell Type
Dendritic cells, Hematopoietic stem and progenitors, Macrophages, Monocytes
Biology Area
Immunology
Molecular Family
CD Molecules
Gene ID
93671 View all products for this Gene ID
UniProt
View information about CD163 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 04/16/2020

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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