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PE anti-mouse CD185 (CXCR5) Recombinant Antibody

Pricing & Availability
Clone
QA20A65 (See other available formats)
Regulatory Status
RUO
Other Names
BLR1, GPCR6, MDR-15
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
QA20A65_PE_CXCR5_Antibody_120522
C57BL/6 mouse splenocytes were stained with anti-mouse CD45R/B220 FITC and anti-mouse CD185 (CXCR5) recombinant (clone QA20A65) PE (left) or rat IgG1, κ PE isotype control (right).
  • QA20A65_PE_CXCR5_Antibody_120522
    C57BL/6 mouse splenocytes were stained with anti-mouse CD45R/B220 FITC and anti-mouse CD185 (CXCR5) recombinant (clone QA20A65) PE (left) or rat IgG1, κ PE isotype control (right).
See PE spectral data
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110503 25 µg $155
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Description

CD185 is also known as CXCR5. It is the receptor for chemokine CXCL13/BLC, which is chemotactic for B cells. CXCR5 is expressed on B cells and a subset of T cells in the spleen, neuronal tissue, lymph nodes, and bone marrow. It is important for migration of B cells into the B cell follicles of the spleen and Peyer’s patches. Follicular helper T cells (Tfh) also express CXCR5 and the ability of these cells to migrate to the lymph node is modulated by the balanced expression of CCR7 and CXCR5.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Recombinant
Host Species
Rat
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
RRID
AB_2927991 (BioLegend Cat. No. 110503)

Antigen Details

Structure
42 kD, seven transmembrane protein
Distribution

B cells and a subset of T cells in mouse spleen, Tfh cells, also expressed in lymph node, bone marrow, and brain

Function
B cell migration into follicles, possible regulatory role in B cell differentiation and Burkitt's lymphoma
Ligand/Receptor
BLC/CXCL13
Cell Type
B cells, T cells, Tfh
Biology Area
Cell Biology, Immunology, Innate Immunity, Neuroinflammation, Neuroscience
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors, GPCR
Antigen References
  1. Kaiser E, et al. 1993. Eur. J. Immunol. 23:2532-9.
  2. Forster R, et al. 1994. Cell. Mol. Biol. 40:381-7.
  3. Forster R, et al. 1994. Blood 84:830-40.
  4. Forster R, et al. 1996. Cell 87:1037-47.
  5. Wolf I, et al. 1998. J. Biol. Chem. 273:28831-6.
Gene ID
12145 View all products for this Gene ID
UniProt
View information about CD185 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/17/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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