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PE anti-mouse CD215 (IL-15Rα) Antibody

Pricing & Availability
Clone
6B4C88 (See other available formats)
Regulatory Status
RUO
Other Names
IL-15RA, IL-15Rα, IL-15 Receptor α subunity
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
6B4C88_PE_CD215_Antibody_103117
Mouse bone marrow derived dendritic cells stimulated overnight with LPS were stained with CD215 (IL-15Rα) PE (clone 6B4C88, filled histogram) or with rat IgG2a, κ isotype control PE (open histogram).
  • 6B4C88_PE_CD215_Antibody_103117
    Mouse bone marrow derived dendritic cells stimulated overnight with LPS were stained with CD215 (IL-15Rα) PE (clone 6B4C88, filled histogram) or with rat IgG2a, κ isotype control PE (open histogram).
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153503 25 µg $159
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153504 100 µg $341
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Description

IL-15 receptor α (IL-15Rα) is a cytokine receptor with the structural homology to IL-2Rα (CD25). Both α receptors contain a group of β sandwich protein called sushi domains. Similar to IL-2Rα, IL-15Rα is able to form a heterotrimer with their β chain (CD122) and common γ chain (γc, CD132). But unlike IL-2Rα subunit, IL-15Rα itself binds to IL-15 with equivalent high affinity compared with the heterotrimeric complex. IL-15Rα is expressed as soluble or transmembrane forms by variety cells, such as activated monocytes, dendritic cells, CD8+ memory T cells, NK or NKT cells. IL-15Rα by itself forms stable complexes with IL-15 on cell surface and presents IL-15 in trans to neighboring cells. IL-15, like IL-2, is a pleiotropic four-helix bundle cytokine and plays a key role in promoting T and B cell survival and proliferation, preferentially in memory CD8+ T cell proliferation, and NK/NKT cell development.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse CD215 recombinant protein (33-205 a.a.)
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Product Citations
  1. Di Pilato M, et al. 2021. Cell. 184(17):4512-4530.e22. PubMed
RRID
AB_2721341 (BioLegend Cat. No. 153503)
AB_2721341 (BioLegend Cat. No. 153504)

Antigen Details

Structure
IL-15 receptor α (IL-15Rα) is a cytokine receptor with the structural homology of IL-2Rα (CD25). Both α receptors contain a group of β sandwich protein called sushi domains.
Distribution

It is found on a variety of subsets including dendritic cells, activated monocytes, memory CD8 T cells and NK/NKT cells.

Function
IL-15Rα is involved in T and B cell proliferation and development, preferentially for memory CD8+ T cell proliferation, and NK/NKT cell development
Ligand/Receptor
IL-15
Cell Type
Dendritic cells, Monocytes, NK cells, NKT cells, T cells
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1. Giri JG, et al. 1995. EMBO J. 14:3654.
2. Giron-Michel J, et al. 2005. Blood. 106:2302.
3. Lorenzen I, et al. 2005. J. Biol. Chem. 281:6642.
4. Sato N, et al. 2007. Proc. Natl. Acad. Sci. USA. 104:588.
5. Anderson BG, Quinn LS. 2016. Endocrinology. 157:1315

Gene ID
16169 View all products for this Gene ID
UniProt
View information about CD215 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 0    Revision Date: 11/01/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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