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PE anti-mouse CD31 Antibody

Pricing & Availability
Clone
MEC13.3 (See other available formats)
Regulatory Status
RUO
Other Names
PECAM-1, EndoCAM
Isotype
Rat IgG2a, κ
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Product Citations
publications
MEC13dot3_PE_072808.jpg
C57BL/6 mouse splenocytes stained with MEC13. 3 PE
  • MEC13dot3_PE_072808.jpg
    C57BL/6 mouse splenocytes stained with MEC13. 3 PE
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102507 50 µg $122
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102508 200 µg $293
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Description

CD31 is a 130-140 kD glycoprotein, also known as platelet endothelial cell adhesion molecule (PECAM-1), EndoCAM, and gpIIa. It is a member of the Ig superfamily, expressed on endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, and T and B cell subsets, and is critical for cell-to-cell interactions. The primary ligands for CD31 have been reported to be CD38 and the vitronectin receptor (αv β3 integrin, CD51/CD61). Other reported functions of CD31 are neutrophil emigration to sites of inflammation, and angiogenesis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Polyoma middle T transformed EC line tEnd.1
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Anti-mouse CD31 clones 390 and MEC13.3 bind to their respective non-overlapping epitopes in IgD2 of CD31.8 Additional reported applications (in the relevant formats) include: immunoprecipitation1, in vitro and in vivo blocking of CD31-mediated cell-cell interactions1-4, immunohistochemical staining1,5,6 of acetone-fixed frozen sections and zinc-fixed paraffin-embedded sections, and spatial biology (IBEX)12,13.
Special Note: The antibody works well on acetone-fixed frozen sections as well as Zinc-fixed paraffin-embedded sections. It sometime works on formalin-fixed and paraformaldehyde-fixed paraffin-embedded tissue sections but inconsistent results have been reported. This antibody is not recommended for formalin-fixed paraffin-embedded sections or for Western blot analysis. The Ultra-LEAF™ Purified antibody (Endotoxin < 0.01 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 102529 and 102530).

Application References

(PubMed link indicates BioLegend citation)
  1. Vecchi A, et al. 1994. Eur. J. Cell Biol. 63:247. (IP, IHC, Block)
  2. Christofidou-Solomidou M, et al. 1997. J. Immunol. 158:4872. (Block)
  3. DeLisser HM, et al. 1997. Am. J. Pathol. 151:671. (Block)
  4. Rosenblum WI, et al. 1994. Am. J. Pathol. 145:33. (Block)
  5. Baldwin HS, et al. 1994. Development 120:2539. (IHC)
  6. Voswinckel R, et al. 2003. Circ. Res. 93:372. (IHC)
  7. Leung VW, et al. 2009. Am J. Pathol. 175:1757. PubMed
  8. Chacko AM, et al. 2012. PLoS One 7:e34958.
  9. Giacomini C, et al. 2014. Exp Eye Res. 18:1. PubMed
  10. Morita R, et al. 2015. PNAS. 112:160. PubMed
  11. Ito A, et al. 2015. Brain Res. 1594:310. PubMed
  12. Radtke AJ, et al. 2020. Proc Natl Acad Sci U S A. 117:33455-65. (SB) PubMed
  13. Radtke AJ, et al. 2022. Nat Protoc. 17:378-401. (SB) PubMed
Product Citations
  1. Naranjo S, et al. 2022. Genes Dev. 36:936. PubMed
  2. Hirano K, et al. 2023. Life Sci Alliance. 6: . PubMed
  3. Michalaki C, et al. 2022. Curr Protoc. 2:e505. PubMed
  4. Vercauteren Drubbel A, et al. 2021. Cell Stem Cell. . PubMed
  5. Hu XF, et al. 2021. Theranostics. 3796:11. PubMed
  6. Kuo A, et al. 2022. Elife. 11:. PubMed
  7. Schloss MJ, et al. 2022. Nat Immunol. 23:605. PubMed
  8. Yoshida H, et al. 2020. Sci Rep. 1.249305556. PubMed
  9. Yanagida K, et al. 2020. Dev Cell. 779:52. PubMed
  10. Khan M, et al. 2017. Sci Rep. 7:41363. PubMed
  11. Higashi AY, et al. 2019. J Am Soc Nephrol. 30:80. PubMed
  12. Takeda K, et al. 2017. Nat Commun. 8:14607. PubMed
  13. Osborne JK, et al. 2021. Cell Reports. 36(3):109408. PubMed
  14. Kusuyama J, et al. 2021. Cell Metabolism. 33(5):939-956.e8. PubMed
  15. Lassen K, et al. 2014. Proc Natl Acad Sci U S A. 111:7741. PubMed
  16. Wang W, et al. 2019. Nat Commun. 10:1580. PubMed
  17. Beyaz S, et al. 2016. Nature. 531:53-58. PubMed
  18. Liu S, et al. 2011. J Natl Cancer Inst. 103:1778. PubMed
  19. Juuri E, et al. 2022. Front Physiol. 13:877565. PubMed
  20. Li P, et al. 2022. EMBO Mol Med. 14:e14511. PubMed
  21. Ye P, et al. 2022. Front Cardiovasc Med. 8:810477. PubMed
  22. Son NH, et al. 2018. J Clin Invest. 128:4329. PubMed
  23. Sefik E, et al. 2021. Nat Biotechnol. . PubMed
  24. Nosho S, et al. 2020. Int J Mol Sci. 21:00. PubMed
  25. Watanabe Y, et al. 2020. J Biol Chem. 295:17632. PubMed
  26. Medler TR et al. 2018. Cancer cell. 34(4):561-578 . PubMed
  27. Hirukawa A, et al. 2019. Cell Rep. 29:249. PubMed
  28. Bota-Rabassedas N, et al. 2021. Cell Reports. 35(3):109009. PubMed
  29. Verginadis II, et al. 2022. Nat Cell Biol. 24:940. PubMed
  30. Sanz-Ros J, et al. 2022. Sci Adv. 8:eabq2226. PubMed
  31. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  32. Gaylo‐Moynihan A et al. 2019. Immunity. 51(2):298-309 . PubMed
  33. Haruwaka K, et al. 2019. Nat Commun. 4.455555556. PubMed
  34. Watanabe Y, et al. 2020. J Biol Chem. . PubMed
  35. Koh BI, et al. 2020. Nat Commun. 3.143055556. PubMed
  36. Lechertier T, et al. 2020. Nat Commun. 2.409722222. PubMed
  37. Liu C, et al. 2022. Nat Commun. 13:1989. PubMed
RRID
AB_312914 (BioLegend Cat. No. 102507)
AB_312914 (BioLegend Cat. No. 102508)

Antigen Details

Structure
Ig superfamily, 130-140 kD
Distribution

Endothelial cells, platelets, granulocytes, monocytes/macrophages, dendritic cells, T and B cell subsets

Function
Adhesion
Ligand/Receptor
CD38, αV/β3 integrin
Cell Type
B cells, Dendritic cells, Endothelial cells, Granulocytes, Macrophages, Monocytes, Neutrophils, Platelets, T cells
Biology Area
Angiogenesis, Cell Adhesion, Cell Biology, Immunology, Neuroinflammation, Neuroscience
Molecular Family
Adhesion Molecules, CD Molecules
Antigen References

1. Barclay AN, et al. 1997. The Leukocyte Antigen FactsBook Academic Press.
2. DeLisser HM, et al. 1994. Immunol. Today 15:490.
3. Newman PJ, et al. 1990. Science 247:1219.

Gene ID
18613 View all products for this Gene ID
UniProt
View information about CD31 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 07/19/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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