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PE anti-mouse CD314 (NKG2D) Antibody

Pricing & Availability
Clone
C7 (See other available formats)
Regulatory Status
RUO
Other Names
CD314
Isotype
Armenian Hamster IgG
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Product Citations
publications
C7
C57BL/6 splenocytes stained with C7 PE and NK1.1 FITC
  • C7
    C57BL/6 splenocytes stained with C7 PE and NK1.1 FITC
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115705 50 µg $122
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Description

NKG2D is a lectin-like type II transmembrane protein also known as CD314. It is expressed on NK cells, a subset of CD8+ T cells, γ/δ T cells and NK1.1+ T cells, as well as in vitro induced LAK cells. NKG2D serves as a stimulatory immunoreceptor to activate NK cells via the non-covalently associated DAP10 or DAP12 adaptor. Several molecules have been identified as the ligands for NKG2D, including minor histocompatibility molecule, H60, UL16-binding protein-like transcript 1 (Mult1, and a family of retinoic acid early transcript 1 (Rae1) in mice, MHC class-I chain-related protein A (MICA), MICB, and UL16-binding proteins (ULBPs) in humans. present in both mice and humans. NKG2D ligands trigger cytokine (IFN-γ, GM-CSF, TNF-α, MIP1β and others) and granzyme release from NK cells. The C7 antibody has been reported to block NK cell killing of targets expressing NKG2D ligands and induce redirected lysis.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Armenian Hamster
Immunogen
Soluble mouse NKG2D
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

Additional reported (for the relevant formats) applications include: blocking1,2,4,5 and inducing redirected cell lysis1,2. To reduce non-specific binding to cells bearing Fc-receptors, pre-incubation of cells with anti-mouse CD16/CD32, clone 93 (Cat. No. 101301/101302), is recommended prior to immunofluorescent staining. For most successful immunofluorescent staining results, it may be important to maximize signal over background by using a relatively bright fluorochrome-antibody conjugate (Cat. No. 115706) or by using a high sensitivity, three-layer staining technique (e.g., including a biotinylated antibody (Cat. No. 115704) or biotinylated anti-Armenian hamster IgG (Cat. No. 405501) second step, followed by SAv-PE (Cat. No. 405204)). The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. No. 115713 & 115714).

Application References

(PubMed link indicates BioLegend citation)
  1. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150. (Block)
  2. Ho EL, et al. 2002. J. Immunol. 169:3667. (Block)
  3. Maeda M, et al. 2004. J. Immunol. 172:6115. (FC)
  4. Andoniou CE, et al. 2005. Nature Immunology 6:1011. (Block)
  5. O'Leary JG, et al. 2006. Nature Immunology 7:507. (Block)
  6. Tang X, et al. 2006. J. Immunol. 177:7645. (FC)
Product Citations
  1. Song Y, et al. 2020. Front Immunol. 11:558143. PubMed
  2. Markov SD, et al. 2021. Mol Cancer Ther. 20:2457. PubMed
  3. Hu J, et al. 2022. J Immunother Cancer. 10:. PubMed
RRID
AB_2133292 (BioLegend Cat. No. 115705)

Antigen Details

Structure
C-type lectin
Distribution

NK and NK-T cells

Function
NK cytolytic killing of target cells expressing NKG2D ligands, costimulation NK cells
Ligand/Receptor
Minor histocompatibility molecule H60, retinoic acid early inducible 1 (RAE-1) family
Cell Type
NK cells, NKT cells
Biology Area
Costimulatory Molecules, Immunology
Molecular Family
CD Molecules
Antigen References

1. Vance RE, et al. 1999. J. Exp. Med. 190:1801.
2. Vance RE, et al. 1998. J. Exp. Med. 188:1841.
3. Lohwasser S, et al. 1999. Eur. J. Immunol. 29:755.
4. Yokoyama WM. 1998. Curr. Opin. Immunol. 10:298.
5. Gilfillan S, et al. 2002. Nat. Immunol. 3:1150.
6. Ho EL, et al. 2002. J. Immunol. 169:3667.

Gene ID
27007 View all products for this Gene ID
UniProt
View information about CD314 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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