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PE anti-mouse Folate Receptor β (FR-β) Antibody

Pricing & Availability
Clone
10/FR2 (See other available formats)
Regulatory Status
RUO
Other Names
FR-β
Isotype
Rat IgG2a, κ
Ave. Rating
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Product Citations
publications
10slashFR2_PE_Folate_Receptorbeta_Antibody_1_110617
Thioglycolate elicited BALB/c mouse peritoneal macrophages were stained with CD14 FITC and anti-mouse Folate Receptor β (clone 10/FR2) PE (top) or PE Rat IgG2a, κ isotype control (bottom).
  • 10slashFR2_PE_Folate_Receptorbeta_Antibody_1_110617
    Thioglycolate elicited BALB/c mouse peritoneal macrophages were stained with CD14 FITC and anti-mouse Folate Receptor β (clone 10/FR2) PE (top) or PE Rat IgG2a, κ isotype control (bottom).
  • 10slashFR2_PE_Folate_Receptorbeta_Antibody_2_110617
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153303 25 µg $141
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153304 100 µg $335
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Description

Folate receptor beta (FR-β) is a member of a family of folate binding receptors that have diverse structural identities but mediate transport of folates into cells. FR-β is a GPI-anchored protein with high affinity for folic acid. It is postulated that this receptor function as folate scavengers when folate supply is low or rapid cell growth requires elevated uptake of folate for methylation reactions including DNA biosynthesis. Although this protein was originally thought to be specific to placenta, it can also exist in other tissues, and it may play a role in the transport of methotrexate in synovial macrophages in rheumatoid arthritis patients.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse Folate receptor β transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Nagai T, et al. 2009. Cancer Immunol Immunother. 1577-86.
Product Citations
  1. Lu JC, et al. 2024. Nat Commun. 621:15. PubMed
  2. Utz SG, et al. 2020. Cell. 181(3):557-573. PubMed
  3. Scheyltjens I, et al. 2022. Nat Protoc. 17:2354. PubMed
RRID
AB_2721343 (BioLegend Cat. No. 153303)
AB_2721343 (BioLegend Cat. No. 153304)

Antigen Details

Structure
30 kD, seven disulfide bonds
Distribution

Placenta, monocytes, tissue resident macrophages

Function
Transport of folate into cells
Ligand/Receptor
Folic Acid
Cell Type
Macrophages, Monocytes
Antigen References

1. Elwood PC, 1989, J Biol Chem, 264:14893.
2. Shen F, et al, 1994, Biochemistry, 8;33:1209.
3. Yi YS, 2016, Immune Netw,16:337.

Gene ID
14276 View all products for this Gene ID
UniProt
View information about Folate Receptor beta on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 0    Revision Date: 11/06/2017

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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