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PE anti-mouse H-2Kb bound to SIINFEKL Antibody

Pricing & Availability
Clone
25-D1.16 (See other available formats)
Regulatory Status
RUO
Other Names
MHC class I molecule Kb bound to the peptide SIINFEKL (Kb-SIINFEKL) H-2Kb, OVA agonist peptide (OVAp), OVA-Kb, OVA257-264 (SIINFEKL)-H-2kb, OVA257-264-H-2kb
Isotype
Mouse IgG1, κ
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Product Citations
publications
25-D1.16_PE_050411
C57BL/6 mouse splenocytes were pulsed with (filled histogram) or without (cyan open histogram) SIINFEKL for 2 hours and then stained with 25-D1.16 PE or mouse IgG1, κ PE isotype control (broken line open histogram).
  • 25-D1.16_PE_050411
    C57BL/6 mouse splenocytes were pulsed with (filled histogram) or without (cyan open histogram) SIINFEKL for 2 hours and then stained with 25-D1.16 PE or mouse IgG1, κ PE isotype control (broken line open histogram).
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141603 25 µg $110
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141604 100 µg $360
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Description

This antibody has been proven to be very useful in tracking the quantity and localization of these specific antigen-presenting cells (APC) in vivo.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
SIINFEKL pulsed RMA-S cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 0.25 µg per million cells in 100 µL volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The 25-D1.16 monoclonal antibody specifically reacts with ovalbumin-derived peptide SIINFEKL bound to H-2Kb of MHC class I, but not with unbound H-2Kb or H-2Kb bound with an irrelevant peptide.  Additional reported applications (for relevant formats) include: immunofluorescence microscopy2,3, immunohistochemical staining of frozen tissue sections3 , and inhibition of T cell response to H-2Kb-SIINFEKL in vitro.

Application References

(PubMed link indicates BioLegend citation)
  1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78. 
  2. Dolan BP, et al. 2010. J. Immunol. 184:1419. (IF)
  3. Porgador A, et al. 1997. Immunity 6:715. (IF, IHC)
  4. Herve J, et al. 2013. J. Immunol. 190:3163. PubMed.
Product Citations
  1. Dong MB, et al. 2020. Cell. 178(5):1189-1204.e23.. PubMed
  2. Abusarah J, et al. 2021. Cell Rep Med. 2:100455. PubMed
  3. Chaurio RA, et al. 2022. Immunity. 55:115. PubMed
  4. Jia JJ, et al. 2022. J Leukoc Biol. 112:157. PubMed
  5. Anadon CM, et al. 2022. Cancer Cell. 40:545. PubMed
  6. Yu Z, et al. 2022. Biomater Sci. 11:263. PubMed
  7. Deák P, et al. 2022. Cell Rep. 41:111563. PubMed
  8. Kaczanowska S, et al. 2021. Cell. 184(8):2033-2052.e21. PubMed
  9. Sethumadhavan S, et al. 2017. PLoS One. . 10.1371/journal.pone.0187314. PubMed
  10. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 5:64. PubMed
  11. Hu CF, et al. 2021. Vaccines (Basel). 9: . PubMed
  12. Zhou L, et al. 2020. Clin Cancer Res. 26:290. PubMed
  13. Walter F, et al. 2020. PLoS One. 15:e0239369. PubMed
  14. Klatt MG, et al. 2020. J Proteomics. 228:103921. PubMed
  15. Guo J, et al. 2019. PLoS One. 14:e0219829. PubMed
  16. Jiang Y, et al. 2020. Adv Mater. 32:e2001808. PubMed
  17. Dai R, et al. 2021. Front Immunol. 12:727046. PubMed
  18. Hervé J, et al. 2013. J Immunol. 190:3163. PubMed
  19. Mohamed E, et al. 2020. Immunity. 52(4):668-682.e7.. PubMed
  20. Gong N, et al. 2020. Nat Nanotechnol. 1.35625. PubMed
  21. Xu DD, et al. 2020. Vaccines (Basel). 8:00. PubMed
  22. Xia Y, et al. 2018. Cell. 175:1059. PubMed
  23. Baumann D, et al. 2020. Nat Commun. 1.969444444. PubMed
  24. Yin X, et al. 2020. Cell Rep. 33:108278. PubMed
  25. Kataru RP, et al. 2019. Cancer Immunol Res. 7:1345. PubMed
  26. Van Den Eeckhout B, et al. 2020. NPJ Vaccines. 0.252777778. PubMed
  27. Song S, et al. 2021. Front Immunol. 12:705140. PubMed
  28. Hong Y, et al. 2019. J Extracell Vesicles. 8:1670893. PubMed
  29. Zhou Q, et al. 2016. Sci Rep. 6:39071. PubMed
RRID
AB_10895905 (BioLegend Cat. No. 141603)
AB_10895905 (BioLegend Cat. No. 141604)

Antigen Details

Structure
OVA peptide257-264 and H-2Kb complex
Distribution

SIINFEKL bond H-2Kb bearing cells

Biology Area
Immunology, Innate Immunity
Molecular Family
MHC Antigens
Antigen References

1. Mareeva T, et al. 2010. J. Immunol. Methods 353:78.
2. Mareeva T, et al. 2008. J. Biol. Chem. 283:29053.
3. Deng Y, et al. 1998. J. Immunol. 161:1677.

Gene ID
14972 View all products for this Gene ID
UniProt
View information about H-2Kb bound to SIINFEKL on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 2    Revision Date: 06/17/2024

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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