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PE anti-mouse/human GL7 Antigen (T and B cell Activation Marker) Antibody

Pricing & Availability
Clone
GL7 (See other available formats)
Regulatory Status
RUO
Other Names
Ly77, T and B cell activation marker
Isotype
Rat IgM, κ
Ave. Rating
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Product Citations
publications
GL7_PE_GL7_Antibody_FC_1_120213
BALB/c mouse bone marrow cells were stained with anti-mouse IgD APC and anti-mouse GL7 (clone GL7 PE (top) or rat IgM, κ PE isotype control (bottom).
  • GL7_PE_GL7_Antibody_FC_1_120213
    BALB/c mouse bone marrow cells were stained with anti-mouse IgD APC and anti-mouse GL7 (clone GL7 PE (top) or rat IgM, κ PE isotype control (bottom).
  • GL7_PE_GL7_Antibody_FC_2_120213
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144607 25 µg $104
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144608 100 µg $262
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Description

The GL7 antigen, also known as Ly77, is a 35 kD protein.  The GL7 antigen has an epitope containing non-sulfated α2-6-sialyl-LacNAc recognized by the GL7 antibody. The GL7 antigen is expressed by pre-B and immature B cells, activated T and B cells, and about 20% of TCR-bright thymocytes.  It is upregulated on mouse splenocytes following activation.  It may play a role in regulation or adhesion.  GL7 high-expressing B cells show higher antibody production and antigen presenting capacity.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
LPS activated DBA/J mouse B cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

The GL7 antibody does not block the binding of CD22 with sulfated a2-6-sialyl-LacNAc.

Cross-reactivity to ferret has been reported by a collaborator, but not verified in house.

Application References

(PubMed link indicates BioLegend citation)
  1. Laszlo G, et al. 1993. J. Immunol. 150:5252. (FC, IP)
  2. Hartgring SA, et al. 2012. Arthritis Res. Ther. 14:R137. (FC)
  3. Taylor JJ, et al. 2012. J. Exp. Med. 209:597. (FC, IHC)
  4. Balogh A, et al. 2010. Immunol. Lett. 130:89. (IHC)
  5. Kimura N, et al. 2007. J. Biol. Chem. 282:32200. (ELISA, FC)
Product Citations
  1. Su C, et al. 2022. NPJ Vaccines. 7:168. PubMed
  2. Zhang B, et al. 2023. Signal Transduct Target Ther. 8:28. PubMed
  3. Zhang YN, et al. 2023. Nat Commun. 14:1985. PubMed
  4. Jing L, et al. 2022. Front Immunol. 13:864995. PubMed
  5. Zhang X, et al. 2021. Front Immunol. 12:680068. PubMed
  6. Kania AK, et al. 2021. Immunohorizons. 5:918. PubMed
  7. Abbott RK, et al. 2018. Immunity. 48:133. PubMed
  8. Huang D, et al. 2020. Nat Commun. 4.520833333. PubMed
  9. He Z, et al. 2016. Exp Hematol. 44:161-165. PubMed
  10. Yang BH, et al. 2020. Cell Reports. 27(12):3629-3645.e6.. PubMed
  11. Shiozawa S, et al. 2022. iScience. 25:103537. PubMed
  12. Chen RJ, et al. 2022. iScience. 25:105595. PubMed
  13. Zhang YN, et al. 2021. Sci Adv. 7:eabj3107. PubMed
  14. Teo TH, et al. 2018. Nat Commun. 9:3905. PubMed
  15. Sanchez HN, et al. 2020. Nat Commun. 0.5. PubMed
  16. Andersen TK, et al. 2021. Front Immunol. 12:747032. PubMed
  17. Vijay R, et al. 2021. J Exp Med. 218:. PubMed
  18. Shimba A et al. 2018. Immunity. 48(2):286-298 . PubMed
  19. Szodoray P, et al. 2021. Cell Reports. 36(6):109525. PubMed
  20. Vijay R, et al. 2020. Nat Immunol. 21:790. PubMed
  21. Ryg-Cornejo V, et al. 2016. Cell Rep. 14:68-81. PubMed
  22. Qi H, et al. 2020. Cell Reports. 31(6):107621. PubMed
RRID
AB_2562925 (BioLegend Cat. No. 144607)
AB_2562925 (BioLegend Cat. No. 144608)

Antigen Details

Structure
35 kD
Distribution

Germinal center B cells, activated B and T cells

Function
Upregulated on activated B cells via in situ repression of CMP-Neu5Ac-hydroxylase
Ligand/Receptor
Neu5Ac-recognizing lectins
Cell Type
B cells, T cells
Biology Area
Immunology, Innate Immunity
Molecular Family
CD Molecules
Antigen References

1. Laszlo G, et al. 1993. J. Immunol. 150:5252.
2. Hathcock KS, et al. 1995. J. Immunol. 155:4575.
3. Cervenak K, et al. 2001. Immunol. Lett. 78:89.

Gene ID
NA
UniProt
View information about GL7 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 12/13/2013

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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