- Clone
- MAR1-5A3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IFNAR1, IFN-αR1, IFN-α/β Receptor Subunit 1
- Isotype
- Mouse IgG1, κ
- Ave. Rating
- Submit a Review
- Product Citations
- publications
Cat # | Size | Price | Quantity Check Availability | Save | ||
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127311 | 25 µg | $134 | ||||
127312 | 100 µg | $292 |
IFNAR-1, the type I IFN receptor subunit 1, is coexpressed with IFNAR-2 on nearly all cells and make up the heterodimeric receptor complex that binds to all type I IFNs (IFN-α/β). Type I IFNs are a group of structurally and functionally related cytokines that have been shown to promote anti-viral, anti-microbial, anti-tumor, and autoimmune responses. Ligand binding to the IFN-α/β receptor complex leads to the tyrosine phosphorylation and activation of IFNAR-1-associated Tyk2 and IFNAR-2-associated Jak1 signal transductions.
Product DetailsProduct Details
- Verified Reactivity
- Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Exogenously expressed murine IFNAR1 extracellular domain
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
- Concentration
- 0.2 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Excitation Laser
-
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
- Application Notes
-
Additional reported applications (for the relevant formats) include: Western blotting1,2, immunoprecipitation1, ELISA1, and blocking8,9. For in vivo studies or highly sensitive assays, we recommend Ultra-LEAF™ purified antibody (Cat. No. 127322) (Endotoxin <0.01 EU/µg).
-
Application References
(PubMed link indicates BioLegend citation) -
- Sheehan KC, et al. 2006. J. Interferon Cytokine Res. 26:804. (FC, Block, IP, WB, ELISA)
- Dunn GP, et al. 2005. Nat. Immunol. 6:722. (FC, WB)
- Miller JC, et al. 2008. J. Immunol. 181:8492. PubMed
- Habjan M, et al. 2009. J. Virol. 83:4365. PubMed
- Kelly-Scumpia KM, et al. 2010. J. Exp. Med. 207:319. PubMed
- Swanson CL, et al. 2010. J. Exp. Med. 207:1485. PubMed
- Marshall HD, et al. 2011. J Virol. epub. PubMed
- Swann JB, et al. 2007. J. Immunol. 178:7540. (Block)
- Jones GW, et al. 2011. FASEB J. 25:409. (Block)
- Mitzel DN, et al. 2014. J. Immunol. 192:4273. PubMed
- Product Citations
-
- RRID
-
AB_1134011 (BioLegend Cat. No. 127311)
AB_1134011 (BioLegend Cat. No. 127312)
Antigen Details
- Distribution
-
IFNAR1 and IFNAR2 are coexpressed on nearly all cells.
- Ligand/Receptor
- IFN-α/β
- Antigen References
-
1. Branca AA, et al. 1981. Nature 294:768.
2. Orchansky P, et al. 1984. J. Interferon Res. 4:275.
3. Hemmi S, et al. 1994. Cell 76:803.
4. Novick D, et al. 1994. Cell 77:391. - Gene ID
- 15975 View all products for this Gene ID
- UniProt
- View information about IFNAR-1 on UniProt.org
Related Pages & Pathways
Pages
Related FAQs
- What type of PE do you use in your conjugates?
- We use R-PE in our conjugates.
Other Formats
View All IFNAR-1 Reagents Request Custom ConjugationDescription | Clone | Applications |
---|---|---|
Purified anti-mouse IFNAR-1 | MAR1-5A3 | FC,IP,WB,ELISA,Block |
Biotin anti-mouse IFNAR-1 | MAR1-5A3 | FC,IP,WB,ELISA |
PE anti-mouse IFNAR-1 | MAR1-5A3 | FC |
APC anti-mouse IFNAR-1 | MAR1-5A3 | FC |
Ultra-LEAF™ Purified anti-mouse IFNAR-1 | MAR1-5A3 | FC,IP,WB,ELISA,Block |
PE/Cyanine7 anti-mouse IFNAR-1 | MAR1-5A3 | FC |
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Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-mouse IFNAR-1
C57BL/6 splenocytes stained with purified MAR1-5A3, followed... -
Biotin anti-mouse IFNAR-1
C57BL/6 mouse splenocytes stained with biotinylated MAR1-5A3... -
PE anti-mouse IFNAR-1
C57BL/6 splenocytes stained with MAR1-5A3 PE -
APC anti-mouse IFNAR-1
C57BL/6 splenocytes stained with MAR1-5A3 APC -
Ultra-LEAF™ Purified anti-mouse IFNAR-1
C57BL/6 splenocytes stained with LEAF™ purified MAR1-5A3, fo... -
PE/Cyanine7 anti-mouse IFNAR-1
Mouse splenocyte were stained with anti-mouse IFNAR-1 (clone...
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