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PE anti-mouse IL-21R Antibody

Pricing & Availability
Clone
4A9 (See other available formats)
Regulatory Status
RUO
Other Names
IL-21 Receptor
Isotype
Rat IgG2a, κ
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Product Citations
publications
4A9_PE_121508.jpg
C57BL-6 splenocytes stained with 4A9 PE
  • 4A9_PE_121508.jpg
    C57BL-6 splenocytes stained with 4A9 PE
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Cat # Size Price Quantity Check Availability Save
131905 25 µg $73
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131906 100 µg $209
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Description

The IL-21R is a member of the cytokine receptor family that closely resembles IL-2R beta and IL-4R alpha. IL-21R is expressed by all mature lymphocytes and up-regulated after B and/or T cell activation, with the highest expression by activated B cells. A functional IL-21R consists of the IL-21R subunit and the commone gamma-chaiin. Upon binding to IL-21, it activates Jak-1, Jak-3, STAT1, STAT3 and STAT 5 transduction pathway.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Rat YB2/0 hybridoma transfectant cell line expressing truncated IL-21R
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per 106 cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Jin H, et al. 2004. J. Immunol. 173:657. (FC)
  2. Jin H and Malek T. 2006. J. Leukocyte Biol. 80:1416. (FC IP)
Product Citations
  1. Fang D, et al. 2022. Immunity. 55:639. PubMed
  2. Chen HY, et al. 2022. Elife. 11:. PubMed
  3. Tian D, et al. 2019. Nat Commun. 10:4246. PubMed
  4. McPhee C, et al. 2013. J Immunol. 191:4581. PubMed
  5. Huang Y, et al. 2016. Innate Immunity. 22: 588 - 597. PubMed
  6. Nagashima H, et al. 2016. J Immunol. 196: 4082 - 4089. PubMed
RRID
AB_1279430 (BioLegend Cat. No. 131905)
AB_1279430 (BioLegend Cat. No. 131906)

Antigen Details

Structure
A member of the class 1 cytokine receptor family. Show significant sequence and structural homology with the IL-4R alpha chain.
Distribution

Express on all mature lymphocytes including B cells, T cells, and NK cells. Reported to be up-regulated upon activation and highest expression on follicular B cells.

Ligand/Receptor
IL-21
Cell Type
B cells, Lymphocytes
Biology Area
Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References

1.Mehta DS, et al. 2004. Immu Reviews 202:84

Gene ID
60504 View all products for this Gene ID
UniProt
View information about IL-21R on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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