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PE anti-mouse Ki-67 Antibody

Pricing & Availability
Clone
16A8 (See other available formats)
Regulatory Status
RUO
Other Names
KIA, proliferation-related Ki-67 antigen
Isotype
Rat IgG2a, κ
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Product Citations
publications
16A8_PE_Ki67_Antibody_ICFC_082112
C57BL/6 mouse splenocytes were stimulated for 3 days with Con-A plus IL-2; subsequently, the cells were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 16A8) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
  • 16A8_PE_Ki67_Antibody_ICFC_082112
    C57BL/6 mouse splenocytes were stimulated for 3 days with Con-A plus IL-2; subsequently, the cells were fixed and permeabilized with 70% ethanol, and then stained with Ki-67 (clone 16A8) PE (filled histogram) or rat IgG2a, κ PE isotype control (open histogram).
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652403 25 µg $153
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652404 100 µg $360
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Description

The nuclear protein Ki-67 was first identified by the monoclonal antibody Ki-67, which was generated by immunizing mice with nuclei of the L428 Hodgkin lymphoma cell line. Ki-67 protein plays an essential role in ribosomal RNA transcription and cell proliferation. Expression of Ki-67 occurs during G1, S, G2, and M phase, while in G0 phase the Ki-67 protein is not detectable. Ki-67 is strongly expressed in proliferating cells and has been reported as a prognostic marker in various tumors.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
E. coli expressed partial mouse Ki-67 recombinant protein, 1816-2163 aa.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by our Ki-67 staining protocol below. For flow cytometric staining, the suggested use of this reagent is ≤0.5 µg per million cells in 100 µl volume.  It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
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  3. Sharma SK, et al. 2015. J Immunol. 194:5529. PubMed
  4. Rodero MP, et al. 2014. J. Invest. Dermatol. 7:1991-7. PubMed
Product Citations
  1. Chan S, et al. 2022. Nat Cancer. . PubMed
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  7. Lucas B, et al. 2023. Nat Commun. 14:2066. PubMed
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  13. Wu M, et al. 2021. Nat Commun. 12:3500. PubMed
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  18. Sekiya T et al. 2018. Cell reports. 24(6):1627-1638 . PubMed
  19. Feng Y, et al. 2018. Kidney Dis (Basel). 4:95. PubMed
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  22. Chen X et al. 2017. Cell stem cell. 21(6):747-760 . PubMed
  23. Schneider MK, et al. 2018. Physiol Rep. 6:e13645. PubMed
  24. Giannini S, et al. 2020. Nat Commun. 0.705555556. PubMed
  25. Bayik D, et al. 2020. Cancer Discov. 1.256944444. PubMed
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  30. Godavarthy PS, et al. 2019. Haematologica. 104(10). PubMed
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  34. Nagatake T, et al. 2018. J Allergy Clin Immunol. 142:470. PubMed
  35. Lehrke MJ, et al. 2021. Elife. 10:. PubMed
  36. King R, et al. 2021. Sci Adv. 7:eabj5293. PubMed
  37. Chakraborty M, et al. 2021. Cell Reports. 34(2):108609. PubMed
  38. Chen Z, et al. 2020. Cell Death Dis. 11:180. PubMed
  39. Sun X, et al. 2021. Nature. 599:673. PubMed
  40. Zhao X, et al. 2022. iScience. 25:104690. PubMed
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  42. Chen Y, et al. 2022. Nat Commun. 13:4468. PubMed
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  45. Kim GC, et al. 2020. Front Oncol. 10:642. PubMed
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RRID
AB_2561524 (BioLegend Cat. No. 652403)
AB_2561524 (BioLegend Cat. No. 652404)

Antigen Details

Structure
325 kD protein containing a forkhead-associated domain (FHA) and 13 tandem repeats
Distribution

Nucleus and chromosome

Function
Required for cell cycle progression and proliferation
Interaction
Interacts with KIF15; binds to MKI67IP through FHA domain
Biology Area
Cell Biology, Cell Cycle/DNA Replication, Transcription Factors
Molecular Family
Nuclear Markers
Antigen References

1. Starborg M, et al. 1996. J. Cell. Sci. 109:143.
2. Byeon IJ, et al. 2005. Nat. Struct. Mol. Biol. 12:987.
3. Yerushalmi R, et al. 2010. Lancet. Oncol. 11:174.
4. Beltrami AP, et al. 2001. N. Engl. J. Med. 344:1750.
5. Sachsenberg N, et al. 1998. J. Exp. Med. 187:1295.
6. Nagy Z, et al. 1997. Acta. Neuropathol. 93:294.

Gene ID
17345 View all products for this Gene ID
UniProt
View information about Ki-67 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 4    Revision Date: 01/08/2016

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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