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PE anti-mouse Tim-4 Antibody

Pricing & Availability
Clone
F31-5G3 (See other available formats)
Regulatory Status
RUO
Other Names
T cell immunoglobulin and mucin domain containing protein-4
Isotype
Rat IgG1, κ
Ave. Rating
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Product Citations
publications
F31-5G3_PE_100908.jpg
BALB/c peritoneal macrophages stained with F31-5G3 PE and M1/70 (CD11b) FITC
  • F31-5G3_PE_100908.jpg
    BALB/c peritoneal macrophages stained with F31-5G3 PE and M1/70 (CD11b) FITC
See PE spectral data
Cat # Size Price Quantity Check Availability Save
129905 25 µg $122
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129906 100 µg $323
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Description

TIM4 is a transmembrane protein known as T cell immunoglobulin and mucin domain containing protein-4. It is expressed on antigen-presenting cells and not on T cells.TIM4 binds to TIM1 to promote T cell proliferation by enhancing cell division and reducing apoptosis.TIM4 bind to phosphatidylserine through its FG-CC’ binding cleft in the N-terminal IgV domain to facilitate the clearance of apoptotic cells or bodies.

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Mouse TIM4-Ig fusion protein
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
0.2 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤0.25 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application References

(PubMed link indicates BioLegend citation)
  1. Rodriguez-Manzanet R, et al. 2008. J. Immunol. 180:4706
Product Citations
  1. De Schepper S et al. 2018. Cell. 175(2):400-415 . PubMed
  2. Samarchith P Kurup et al. 2019. Cell host & microbe. 25(4):565-577 . PubMed
RRID
AB_1227799 (BioLegend Cat. No. 129905)
AB_1227799 (BioLegend Cat. No. 129906)

Antigen Details

Structure
Transmembrane protein containing immunoglobulin domain and mucin-like domain
Distribution

Unlike the other TIMs, TIM4 is only expressed by antigen-presenting cells, but not by T cells.

Function
TIM4 is expressed on antigen-presenting cells. It provides co-stimulation to promote T cell proliferation and survival and has a possible role in clearance of apoptotic cells by binding phosphatidylserine.
Interaction
Phosphatiylserine is the ligand of TIM4 and TIM4 is a ligand of TIM1
Ligand/Receptor
Phosphatidylserine, TIM1
Cell Type
Antigen-presenting cells
Biology Area
Immunology
Antigen References

1. Kuchroo VK, et al. 2008. Nat. Rev. Immunol. 8:577
2. Miyanishi M et al. 2007. Nature 450:435
3. Rodriguez-Manzanet R et al. 2008. J. Immunol. 180:4706

Gene ID
276891 View all products for this Gene ID
UniProt
View information about Tim-4 on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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