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PE anti-Puromycin Antibody

Pricing & Availability
Clone
2A4 (See other available formats)
Regulatory Status
RUO
Other Names
Puromycin from streptomyces alboniger
Isotype
Mouse IgG2a, κ
Ave. Rating
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Product Citations
publications
2A4_PE_Puromycin_Antibody_1_041222
The human pro-monocytic, human myeloid leukemia cell line, U937 was treated with Puromycin (1 µg/mL) for 1h (filled histogram) or left untreated (open histogram) and then intracellularly stained with anti-Puromycin (clone 2A4) PE using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803).
  • 2A4_PE_Puromycin_Antibody_1_041222
    The human pro-monocytic, human myeloid leukemia cell line, U937 was treated with Puromycin (1 µg/mL) for 1h (filled histogram) or left untreated (open histogram) and then intracellularly stained with anti-Puromycin (clone 2A4) PE using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803).
  • 2A4_PE_Puromycin_Antibody_2_041222
    Human peripheral blood mononuclear cells were stimulated with Cell Activation Cocktail in the presence of Puromycin (1 µg/mL) (filled, dark purple histogram) or without Puromycin (open histogram) for 2h or left unstimulated (filled, light purple histogram), and then intracellularly stained with anti-Puromycin (clone 2A4) PE using the Cyto-Fast™ Fix/Perm Buffer Set (Cat. No. 426803).
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381503 25 tests $160
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381504 100 tests $369
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Description

Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, which functions as a Tyr-tRNA mimetic that enters the ribosome A site and incorporates into the nascent chain C terminus. Antibodies against Puromycin can detect puromycylated nascent chains released from ribosomes and thereby quantify protein translation. This monoclonal antibody to puromycin can be used in flow cytometry, immunofluorescence or immunoblotting to measure rates of global protein synthesis (mRNA translation) in cells incubated with Puromycin. 

Product Details
Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat, All species
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Puromycin from Streptomyces alboniger coupled to KLH
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µL per million cells in 100 µL staining volume or 5 µL per 100 µL of whole blood. It is recommended that the reagent be titrated for optimal performance for each application.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

It is recommended that the timing and concentration of Puromycin treatment be optimized for each cell type. 1.0 - 10.0 µg/mL of Puromycin for 30 minutes to 2 hours are suggested ranges for activated PBMCs or cell lines in growth phase.

For flow cytometry, the amount of anti-puromycin antibody may also have to be optimized. Cells with high levels of protein translation require lower concentrations of antibody.  

RRID
AB_2927816 (BioLegend Cat. No. 381503)
AB_2927816 (BioLegend Cat. No. 381504)

Antigen Details

Distribution

Cytoplasm, Ribosomes

Cell Type
Embryonic Stem Cells
Biology Area
Cell Biology, Protein Synthesis
Antigen References
  1. Schmidt E, et al. 2009. Nat Methods. 6:275-7.
  2. Arguello R, et al. 2020. Cell Metabolism. 32:1063-1075.
Gene ID
NA
UniProt
View information about Puromycin on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 04/12/2022

For Research Use Only. Not for diagnostic or therapeutic use.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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