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PE anti-rat IFN-γ Antibody

Pricing & Availability
Clone
DB-1 (See other available formats)
Regulatory Status
RUO
Other Names
Interferon-γ, Immune interferon, Type II interferon, T cell interferon, Macrophage-activating factor (MAF), IFN-g, IFN-gamma
Isotype
Mouse IgG1, κ
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Product Citations
publications
DB-1 Rat
PMA/lonomycin stimulated Lou rat splenocytes were stained with DB-1 PE.
  • DB-1 Rat
    PMA/lonomycin stimulated Lou rat splenocytes were stained with DB-1 PE.
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507806 100 tests $238
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Description

Interferon-γ is a potent multifunctional cytokine which is secreted primarily by activated NK cells and T cells. Originally characterized based on anti-viral activities, IFN-γ also exerts anti-proliferative, immunoregulatory, and proinflammatory activities. IFN-γ can upregulate MHC class I and II antigen expression by antigen-presenting cells. The DB-1 antibody reacts with rat and mouse interferon-gamma (IFN-γ). The DB-1 antibody can neutralize the bioactivity of natural or recombinant IFN-γ. The DB-1 antibody has been well characterized for ELISPOT, ELISA, intracellular staining, Western blotting, IHC, and neutralization (in vitro and in vivo).

Product Details
Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Recombinant rat IFN-γ
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and BSA (origin USA)
Preparation
The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions.
Concentration
Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Application

ICFC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by intracellular immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 5 µl per million cells in 100 µl staining volume or 5 µl per 100 µl of whole blood.

Excitation Laser
Blue Laser (488 nm)
Green Laser (532 nm)/Yellow-Green Laser (561 nm)
Application Notes

ELISA Capture1 or ELISPOT Capture2: The purified DB-1 antibody is useful as the capture antibody in a sandwich ELISA or ELISPOT assay, when used in conjunction with the biotinylated poly5109 antibody (Cat. No. 510901) as the detecting antibody and recombinant IFN-? (Cat. No. 565701) as the standard. The LEAF™ purified antibody is suggested for ELISPOT capture.
Flow Cytometry5: The fluorochrome-labeled DB-1 antibody is useful for intracellular immunofluorescent staining and flow cytometric analysis to identify IFN-?-producing cells within mixed cell populations. For intracellular cytokine staining protocol, please visit www.biolegend.com and click on the support section.
Neutralization3,4: The LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for neutralization of rat IFN-? bioactivity in vivo and in vitro (Cat. No. 507808).
Additional reported applications (for the relevant formats) include: Western blotting1, and immunohistochemistry2 of paraformaldehyde-fixed, saponin-treated frozen tissue sections.

Application References

(PubMed link indicates BioLegend citation)
  1. Van der Meide P, et al. 1989. Lymphokine Res. 8:439.
  2. Nennesmo I, et al. 1989. Brain Res. 504:306.
  3. Rayner D, et al. 1987. Scand. J. Immunol. 25:621.
  4. Hartung H, et al. 1990. Ann Neurol. 27:247.
  5. Bernard I, et al. 1998. Eur. Cytokine Net. 9:613.
Product Citations
  1. Wang Z, et al. 2023. Sci Adv. 9:eabo4100. PubMed
  2. Jiang S, et al. 2020. Scand J Immunol. e12867:91. PubMed
  3. Wang Z, et al. 2022. Nat Biomed Eng. 6:791. PubMed
  4. DU T, et al. 2020. Front Immunol. 1.571527778. PubMed
  5. Boehm D et al. 2017. Cell host & microbe. 21(5):569-579 . PubMed
  6. Huang G, et al. 2021. Exp Ther Med. 22:807. PubMed
RRID
AB_315497 (BioLegend Cat. No. 507806)

Antigen Details

Structure
Cytokine; dimer; 40-80 kD (Mammalian)
Bioactivity
Antiviral/antiparasitic activities; inhibits proliferation; enhances MHC class I and II expression on APC
Cell Sources
CD8+ and CD4+ T cells, NK cells
Cell Targets
T cells, B cells, macrophages, NK cells, endothelial cells, fibroblasts
Receptors
IFN-γRα (CDw119) dimerized with IFN-γRβ (AF-1)
Cell Type
Tregs
Biology Area
Immunology
Molecular Family
Cytokines/Chemokines
Antigen References

1. Fitzgerald K, et al. Eds. 2001. The Cytokine FactsBook. Academic Press San Diego.
2. De Maeyer E, et al. 1992. Curr. Opin. Immunol. 4:321.
3. Farrar M, et al. 1993. Annu .Rev. Immunol. 11:571.
4. Gray P, et al. 1987. Lymphokines 13:151.

Regulation
Upregulated by IL-2, bFGF, EGF; downregulated by 1-α-25-Dihydroxy vitamin D3, dexamethasone
Gene ID
25712 View all products for this Gene ID
UniProt
View information about IFN-gamma on UniProt.org

Related FAQs

What type of PE do you use in your conjugates?
We use R-PE in our conjugates.
Go To Top Version: 1    Revision Date: 11/30/2012

For Research Use Only. Not for diagnostic or therapeutic use.

 

This product is supplied subject to the terms and conditions, including the limited license, located at www.biolegend.com/terms) ("Terms") and may be used only as provided in the Terms. Without limiting the foregoing, BioLegend products may not be used for any Commercial Purpose as defined in the Terms, resold in any form, used in manufacturing, or reverse engineered, sequenced, or otherwise studied or used to learn its design or composition without express written approval of BioLegend. Regardless of the information given in this document, user is solely responsible for determining any license requirements necessary for user’s intended use and assumes all risk and liability arising from use of the product. BioLegend is not responsible for patent infringement or any other risks or liabilities whatsoever resulting from the use of its products.

 

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

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